Full Product Name
C/EBP-epsilon (Ab-74) Antibody
Product Synonym Names
C/EBP epsilon; CCAAT/enhancer binding protein epsilon; CEBPE
Product Gene Name
anti-C/EBP-epsilon antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q15744
Specificity
C/EBP-epsilon (Ab-74) antibody detects endogenous levels of total C/EBP-epsilon protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human C/EBP-epsilon around the phosphorylation site of threonine 74 (P-G-TP-P-A).
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-C/EBP-epsilon antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-C/EBP-epsilon antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-C/EBP-epsilon antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-C/EBP-epsilon antibody
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000
Testing Data of anti-C/EBP-epsilon antibody
Peptide-+
Immunohistochemistry (IHC) of anti-C/EBP-epsilon antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue using C/EBP-epsilon (Ab-74) antibody. Western blot analysis of extracts from HuvEc cells, treated with Insulin (0.01U/ml, 15mins), using C/EBP-epsilon (Ab-74) antibody.
Immunofluorescence (IF) of anti-C/EBP-epsilon antibody
Peptide-+ Immunofluorescence analysis of HeLa cells, using C/EBP-epsilon (Ab-74) antibody.
NCBI/Uniprot data below describe general gene information for C/EBP-epsilon. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001796.2
[Other Products]
NCBI GenBank Nucleotide #
NM_001805.3
[Other Products]
UniProt Primary Accession #
Q15744
[Other Products]
UniProt Secondary Accession #
Q15745; Q8IYI2; Q99803[Other Products]
UniProt Related Accession #
Q15744[Other Products]
Molecular Weight
30,603 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein epsilon
NCBI Official Synonym Full Names
CCAAT enhancer binding protein epsilon
NCBI Official Symbol
CEBPE??[Similar Products]
NCBI Official Synonym Symbols
CRP1; C/EBP-epsilon
??[Similar Products]
NCBI Protein Information
CCAAT/enhancer-binding protein epsilon
UniProt Protein Name
CCAAT/enhancer-binding protein epsilon
UniProt Gene Name
CEBPE??[Similar Products]
UniProt Synonym Gene Names
C/EBP epsilon??[Similar Products]
NCBI Summary for C/EBP-epsilon
The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for C/EBP-epsilon
Transcriptional activator (PubMed:26019275). C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).
Product References and Citations for anti-C/EBP-epsilon antibody
Arati Khanna-Gupta. PNAS, Jul 2001; 98: 8000. Dazhong Zhuang. J. Biol. Chem., Apr 2006; 281: 10745-10751. Julie A. Lekstrom-Himes. Stem Cells, Mar 2001; 19: 125-133. Ryuya Yamanaka. PNAS, Nov 1997; 94: 13187.
Research Articles on C/EBP-epsilon
1. CEBPE expression was highest in multipotent progenitor cells (S1) and declined sharply as cells progressed to B-cell-committed progenitors, including pre-B-I cells (S2), pre-B-II cells (S3) and immature B cells (S4)
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
