Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2; JAK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This JAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1101-1132 amino acids from the C-terminal region of human JAK2.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-JAK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Cancer; Cell Biology; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

WB~~1:1000

The anti-JAK2 Pab is used in Western blot to detect JAK2 in mouse thymus tissue lysate.

Confocal immunofluorescent analysis of JAK2 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

JAK2 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

130674

Janus kinase 2

tyrosine-protein kinase JAK2

Tyrosine-protein kinase JAK2

JAK-2??[Similar Products]

JAK2_HUMAN

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490.

Protein type: Oncoprotein; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; TK group; JakA family

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: nucleoplasm; extrinsic to internal side of plasma membrane; cytoskeleton; nuclear matrix; cytoplasm; caveola; nucleus; cytosol; lipid raft

Molecular Function: protein C-terminus binding; histone binding; non-membrane spanning protein tyrosine kinase activity; acetylcholine receptor binding; protein kinase binding; protein kinase activity; interleukin-12 receptor binding; protein binding; growth hormone receptor binding; peptide hormone receptor binding; protein-tyrosine kinase activity; insulin receptor substrate binding; heme binding; phosphoinositide 3-kinase binding; SH2 domain binding; type 1 angiotensin receptor binding; ATP binding; receptor binding

Biological Process: establishment and/or maintenance of chromatin architecture; positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; response to antibiotic; regulation of apoptosis; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; mesoderm development; negative regulation of neuron apoptosis; positive regulation of cell activation; positive regulation of DNA binding; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; positive regulation of tumor necrosis factor production; JAK-STAT cascade; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; mineralocorticoid receptor signaling pathway; response to hydroperoxide; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; apoptosis; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; actin filament polymerization; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cell migration; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; regulation of cell proliferation; positive regulation of interleukin-1 beta production; G-protein coupled receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; innate immune response; negative regulation of cell-cell adhesion; blood coagulation; cell motility; induction of apoptosis by oxidative stress; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Polycythemia Vera; Myelofibrosis; Erythrocytosis, Familial, 1; Budd-chiari Syndrome; Thrombocythemia 3

Joos, S., et al., Int. J. Cancer 103(4):489-495 (2003).
Leung, K.C., et al., Proc. Natl. Acad. Sci. U.S.A. 100(3):1016-1021 (2003).
Saharinen, P., et al., J. Biol. Chem. 277(49):47954-47963 (2002).
Giordanetto, F., et al., Protein Eng. 15(9):727-737 (2002).
Deo, D.D., et al., J. Biol. Chem. 277(24):21237-21245 (2002).

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