For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against IDH2. It has beenselected for its ability to recognize IDH2 in immunohistochemical staining andwestern blotting.

Affinity Chromatography

Liquid

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of anti-IDH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Knock-Out Validation Antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human Jurkat Cells; Lane3: Porcine Heart Tissue; Lane4: Porcine Liver Tissue.

Western Blot: Sample: Recombinant IDH2, Human.

DAB staining on IHC-P; Samples: Human Gliama Tissue.

DAB staining on IHC-P; Samples: Human Liver Cancer Tissue

DAB staining on IHC-P; Samples: Human Stomach Tissue

DAB staining on IHC-P; Samples: Human Kidney Tissue

Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: IDH2 knockout Jurkat cell lysate;;Predicted MW: 51,45kDa ;Observed MW: 45kDa;Primary Ab: 1ug/ml Rabbit Anti-Human IDH2 Antibody;Second Ab: 0.2ug/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)

45,180 Da

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial

isocitrate dehydrogenase [NADP], mitochondrial

Isocitrate dehydrogenase [NADP], mitochondrial

IDH??[Similar Products]

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Mitochondrial; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytosol; extracellular exosome; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; peroxisome

Molecular Function: isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; NAD binding

Biological Process: 2-oxoglutarate metabolic process; carbohydrate metabolic process; glyoxylate cycle; isocitrate metabolic process; mitochondrion organization; NADP biosynthetic process; negative regulation of glial cell migration; negative regulation of matrix metallopeptidase secretion; tricarboxylic acid cycle

Disease: D-2-hydroxyglutaric Aciduria 2

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