Product Name
Isocitrate dehydrogenase [NADP], mitochondrial (IDH2), ELISA Kit
Full Product Name
Human Isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit
Product Synonym Names
Isocitrate dehydrogenase [NADP], mitochondrial; IDH; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2; 1.1.1.42
Product Gene Name
IDH2 elisa kit
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P48735
Detection Range
0.312-20 ng/mL
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IDH2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IDH2 purchase
MBS288993 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IDH2. The ELISA analytical biochemical technique of the MBS288993 kit is based on IDH2 antibody-IDH2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IDH2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IDH2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for IDH2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001276839.1
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NCBI GenBank Nucleotide #
NM_001289910.1
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UniProt Primary Accession #
P48735
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UniProt Secondary Accession #
Q96GT3; B2R6L6; B4DFL2[Other Products]
UniProt Related Accession #
P48735[Other Products]
Molecular Weight
45,180 Da
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 2 (NADP+), mitochondrial
NCBI Official Symbol
IDH2??[Similar Products]
NCBI Official Synonym Symbols
IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
??[Similar Products]
NCBI Protein Information
isocitrate dehydrogenase [NADP], mitochondrial
UniProt Protein Name
Isocitrate dehydrogenase [NADP], mitochondrial
UniProt Synonym Protein Names
ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Protein Family
Isocitrate dehydrogenase
UniProt Gene Name
IDH2??[Similar Products]
UniProt Synonym Gene Names
IDH??[Similar Products]
UniProt Entry Name
IDHP_HUMAN
NCBI Summary for IDH2
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Comments for IDH2
IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.
Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; Other Amino Acids Metabolism - glutathione; EC 1.1.1.42
Chromosomal Location of Human Ortholog: 15q26.1
Cellular Component: cytosol; mitochondrial inner membrane; mitochondrial matrix; mitochondrion; peroxisome
Molecular Function: isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; NAD binding
Biological Process: 2-oxoglutarate metabolic process; carbohydrate metabolic process; cellular metabolic process; glyoxylate cycle; isocitrate metabolic process; tricarboxylic acid cycle
Disease: D-2-hydroxyglutaric Aciduria 2
Research Articles on IDH2
1. data demonstrate important clinical and biological differences between IDH1(MT) and IDH2(MT) myeloid neoplasms
Precautions
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Disclaimer
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