PHKD; CAMII; PHKD2; Calmodulin 2; CALM 2; Calmodulin2; CAM II; LP7057 protein; PHKD 2; Phosphorylase kinase delta; Calmodulin-2; CALM2

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a, k

J4D8

Mouse

By protein-G affinity chromatography

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

1mg/ml (lot specific)

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of anti-CALM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Calmodulin has been known to act as an intracellular calcium sensor protein. When the intracellular Ca2+ concentration increases, calmodulin can bind up to four Ca2+, changing its conformation and regulating cellular functions such as activation or inhibition of a large number of enzymes, ion channels, and receptors.

Signal Transduction

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calmodulin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: A549 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: Ramos cell lysate Lane 7.: PC3 cell lysate

ICC/IF analysis of Calmodulin in HeLa cells. The cell was stained with ACA0830 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

16,838 Da[Similar Products]

calmodulin 2

calmodulin-2

Calmodulin-2

This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).

Zhang M., et al. (1998). Biochem Cell Biol. 76 (2-3):313-23; James.P., et al. (1995). Trend Biochem Sci. 20: 38-42

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