Product Name
FGF-23, Recombinant Protein
Popular Item
Full Product Name
Recombinant Mouse FGF-23
Product Synonym Names
Fibroblast Growth Factor 23
Product Gene Name
FGF-23 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MYPDTSPLLG SNWGSLTHLY TATARTSYHL QIHRDGHVDG TPHQTI YSAL MITSEDAGSV VITGAMTRRF LCMDLHGNIF GSLHFSPENC K FRQWTLENG YDVYLSQKHH YLVSLGRAKR IFQPGTNPPP FSQFLAR RNE VPLLHFYTVR PRRHTRSAED PPERDPLNVL KPRPRATPVP VS CSRELPSA EEGGPAASDP LGVLRRGRGD ARGGAGGADR CRPFPRFV
Chromosome Location
Chromosome: 6; NC_000072.6 (127072902..127082296). Location: 6 F3; 6
3D Structure
ModBase 3D Structure for Q9EPC2
Purity/Purification
> 95% by SDS-PAGE & HPLC analyses
Biological Activity
Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. The expected ED50 for this effect is 0.3-0.5 ug/ml, in the presence of murine Klotho and heparin.
Endotoxin Level
< 0.1 ng/ug of protein (<1EU/ug)
Reconstitution
Centriguge the vial before opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it recommended to further dilute in a buffer containing a carrier protein (example: 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of FGF-23 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FGF-23 recombinant protein
The FGF family plays a central role during prenatal development and postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-23, FGF-21 and FGF-19 constitute an atypical FGF subfamily whose ligands act as circulating hormones and require the participation of a Klotho protein as a co-receptor for their signaling. FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, a Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1. Recombinant murine FGF-23 is a 25.5 kDa globular protein containing 228 amino acid residues.
Product Categories/Family for FGF-23 recombinant protein
Cytokines & Growth Factors
NCBI/Uniprot data below describe general gene information for FGF-23. It may not necessarily be applicable to this product.
NCBI Accession #
NP_073148.1
[Other Products]
NCBI GenBank Nucleotide #
NM_022657.4
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UniProt Primary Accession #
Q9EPC2
[Other Products]
UniProt Related Accession #
Q9EPC2[Other Products]
Molecular Weight
25.5 kDa
NCBI Official Full Name
fibroblast growth factor 23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
Fgf23??[Similar Products]
NCBI Protein Information
fibroblast growth factor 23; FGF-23
UniProt Protein Name
Fibroblast growth factor 23
UniProt Gene Name
Fgf23??[Similar Products]
UniProt Synonym Gene Names
FGF-23??[Similar Products]
UniProt Entry Name
FGF23_MOUSE
NCBI Summary for FGF-23
This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function. [provided by RefSeq, Mar 2013]
UniProt Comments for FGF-23
Function: Regulator of phosphate homeostasis
By similarity. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels
By similarity. Acts directly on the parathyroid to decrease PTH secretion
By similarity. Regulator of vitamin-D metabolism
By similarity. Negatively regulates osteoblasts differentiation and matrix mineralization
By similarity. Upregulates EGR1 expression in the presence of KL. Ref.3
Subunit structure: Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL and heparan sulfate glycosaminoglycans that function as coreceptors
By similarity. Ref.3
Subcellular location: Secreted
By similarity. Note: Secretion is dependent on O-glycosylation
By similarity.
Tissue specificity: Mainly expressed in the brain and thymus at low levels. In brain; preferentially expressed in the ventrolateral thalamic nucleus.
Post-translational modification: Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases
By similarity.O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23
By similarity.
Sequence similarities: Belongs to the heparin-binding growth factors family.
Research Articles on FGF-23
1. The effects of chronic low calcium to prevent 1,25(OH)2D and PTH stimulation of FGF-23 in these mutant mice suggest that suppression of FGF-23 plays an important physiological adaptive response to hypocalcemia.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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