Product Name
CASQ2, siRNA
Full Product Name
CASQ2 siRNA (Mouse)
Product Synonym Names
Calsequestrin-2; Calsequestrin. cardiac muscle isoform
Product Gene Name
CASQ2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O09161
Specificity
CASQ2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CASQ2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CASQ2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CASQ2 sirna
siRNA to inhibit CASQ2 expression using RNA interference
Applications Tested/Suitable for CASQ2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CASQ2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033944.2
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NCBI GenBank Nucleotide #
NM_009814.3
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UniProt Primary Accession #
O09161
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UniProt Secondary Accession #
O88505; Q56A03[Other Products]
UniProt Related Accession #
O09161[Other Products]
Molecular Weight
48,176 Da
NCBI Official Full Name
calsequestrin-2
NCBI Official Synonym Full Names
calsequestrin 2
NCBI Official Symbol
Casq2??[Similar Products]
NCBI Official Synonym Symbols
Csq2; cCSQ; ESTM52; AA033488; AW146219
??[Similar Products]
NCBI Protein Information
calsequestrin-2
UniProt Protein Name
Calsequestrin-2
UniProt Synonym Protein Names
Calsequestrin, cardiac muscle isoform
Protein Family
Calsequestrin
UniProt Gene Name
Casq2??[Similar Products]
UniProt Entry Name
CASQ2_MOUSE
UniProt Comments for CASQ2
calsequestrin 2: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions. Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2); also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. Belongs to the calsequestrin family.
Protein type: Calcium-binding
Cellular Component: sarcoplasmic reticulum lumen; sarcoplasmic reticulum; junctional membrane complex; cytoplasm; intracellular; Z disc
Molecular Function: protein homodimerization activity; protein kinase C binding; metal ion binding; calcium ion binding; calcium-dependent protein binding
Biological Process: protein polymerization; regulation of muscle contraction; regulation of heart rate; sequestering of calcium ion; negative regulation of potassium ion transport; sarcomere organization; cardiac muscle contraction
Research Articles on CASQ2
1. Viral gene transfer of wild-type CASQ2 into the heart of R33Q mice prevents and reverts severe manifestations of catecholaminergic polymorphic ventricular tachycardia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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