Full Product Name
CASQ2 antibody
Product Synonym Names
PDIB2; CASQ2; Calsequestrin 2 (cardiac muscle); Calsequestrin 2 fast twitch cardiac muscle; Calsequestrin cardiac muscle isoform; Calsequestrin fast twitch cardiac muscle; Calsequestrin2; Cardiac calsequestrin 2; CASQ 2; ESTM52; FLJ26321; FLJ93514; PDIB2.
Product Gene Name
anti-CASQ2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O14958
Species Reactivity
Human, Mouse
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
Recombinant human CASQ2 (20-399aa) purified from E. coli
Clone Note
Anti-human CASQ2 mAb, clone AT4E10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CASQ2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of anti-CASQ2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CASQ2 antibody
CASQ2 (Calsequestrin 2) protein responsible for holding Calcium together with other two proteins junction and triadin at the sarcoplasmic reticulum renders the hear vulnerable to severe cardiac arrhythmias, known as CPVT (catecholaminergic polymorphic ventricular tachycardia), during physical exercise.
Product Categories/Family for anti-CASQ2 antibody
Cardiovascular
Applications Tested/Suitable for anti-CASQ2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CASQ2 antibody
WB: 1:1000.
Recommended starting dilution is 1:1000
Western Blot (WB) of anti-CASQ2 antibody
The extracts of Mouse muscle (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CASQ2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence (IF) of anti-CASQ2 antibody
ICC/IF analysis of CASQ2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CASQ2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
NCBI/Uniprot data below describe general gene information for CASQ2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001223
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NCBI GenBank Nucleotide #
NM_001232.3
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UniProt Primary Accession #
O14958
[Other Products]
UniProt Secondary Accession #
Q5T1D2; Q8TBW8; B2R7M6; B4DIB0[Other Products]
UniProt Related Accession #
O14958[Other Products]
Molecular Weight
38,269 Da
NCBI Official Full Name
calsequestrin-2
NCBI Official Synonym Full Names
calsequestrin 2
NCBI Official Symbol
CASQ2??[Similar Products]
NCBI Official Synonym Symbols
PDIB2
??[Similar Products]
NCBI Protein Information
calsequestrin-2
UniProt Protein Name
Calsequestrin-2
UniProt Synonym Protein Names
Calsequestrin, cardiac muscle isoform
Protein Family
Calsequestrin
UniProt Gene Name
CASQ2??[Similar Products]
NCBI Summary for CASQ2
The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]
UniProt Comments for CASQ2
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
Product References and Citations for anti-CASQ2 antibody
Eckstein, A.K., et al. (2006) J. Clin. Endo. and Meta. 91: 3464-3470.; Paschke, R., et al (1995) Clin. Endo. 42: 565-569.; Bahn, R.S. (2003) J. Clin. Endo. and Meta. 88: 1939-1946.
Research Articles on CASQ2
1. We show for the first time a heterozygous CASQ2 variant causing autosomal dominant CPVT in a large family with a severe phenotype.
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