Full Product Name
FTCD siRNA (Mouse)
Product Synonym Names
Formimidoyltransferase-cyclodeaminase; Formiminotransferase-cyclodeaminase; FTCD
Product Gene Name
FTCD sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q91XD4
Specificity
FTCD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FTCD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of FTCD sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
FTCD sirna
siRNA to inhibit FTCD expression using RNA interference
Applications Tested/Suitable for FTCD sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for FTCD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_543121.1
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NCBI GenBank Nucleotide #
NM_080845.2
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UniProt Primary Accession #
Q91XD4
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UniProt Related Accession #
Q91XD4[Other Products]
Molecular Weight
58,939 Da
NCBI Official Full Name
formimidoyltransferase-cyclodeaminase
NCBI Official Synonym Full Names
formiminotransferase cyclodeaminase
NCBI Official Symbol
Ftcd??[Similar Products]
NCBI Protein Information
formimidoyltransferase-cyclodeaminase
UniProt Protein Name
Formimidoyltransferase-cyclodeaminase
UniProt Synonym Protein Names
Formiminotransferase-cyclodeaminase; FTCD
Protein Family
Formimidoyltransferase-cyclodeaminase
UniProt Gene Name
Ftcd??[Similar Products]
UniProt Synonym Gene Names
FTCD??[Similar Products]
UniProt Entry Name
FTCD_MOUSE
UniProt Comments for FTCD
FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Lyase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 4.3.1.4; EC 2.1.2.5; Amino Acid Metabolism - histidine; Methyltransferase
Cellular Component: Golgi apparatus; cytoskeleton; cytoplasm
Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; transferase activity; glutamate formimidoyltransferase activity; lyase activity; catalytic activity; folic acid binding
Biological Process: cellular metabolic process; metabolic process; cytoskeleton organization and biogenesis; histidine metabolic process; histidine catabolic process
Research Articles on FTCD
1. Autoreactive B cell response against formiminotransferase-cyclodeaminase correlated with disease activity, possibly linking B-cell autoreactivity and AIH pathogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
