Product Name
FSH (BRD2), Monoclonal Antibody
Full Product Name
Mouse anti FSH
Product Gene Name
anti-BRD2 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 6; Location: 6p21.3
3D Structure
ModBase 3D Structure for P25440
Specificity
The antibody stains cytoplasma localized Follicle Stimulating Hormone in beta cells in the pars distalis of the pituitary gland.
Form/Format
The vial contains 1 ml diluted Mouse ascites, containing 0.09% sodium azide.
Source Note
1038 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with Human FSH.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Other Notes
Small volumes of anti-BRD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-BRD2 antibody
Follicle Stimulating Hormone (FSH) is produced in the anterior lobe of the pituitary gland. It enables ovarian folliculogenesis to the antral follicle stage and is essential for Sertoli cell prolifeRation and maintenance of sperm quality in the testis. Members of the pituitary glycoprotein hormone family, of which FSH is one (see also luteinizing hormone, chorionic gonadotropin, and thyroid stimulating hormone), consist of a shared alpha chain and a beta chain encoded by a sepaRate gene. The FSHB gene encodes the beta subunit of follicle stimulating hormone.
Product Categories/Family for anti-BRD2 antibody
Hormones
Applications Tested/Suitable for anti-BRD2 antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)
Application Notes for anti-BRD2 antibody
1038 is suitable for immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded (without pretreatment) tissue sections. Recommended antibody dilution: 1:10 based on the indirect immunofluorescence method. The optimal dilution should be tested by serial dilution. Dilute immediately before use with PBS or TRIS-HCl pH 7.0.
NCBI/Uniprot data below describe general gene information for BRD2. It may not necessarily be applicable to this product.
UniProt Primary Accession #
P25440
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UniProt Secondary Accession #
O00699; O00700; Q15310; Q5STC9; Q6P3U2; Q969U4; B0S7P0; B1AZT1[Other Products]
UniProt Related Accession #
P25440; Q63HQ9; Q658Y7; Q9UNM9[Other Products]
Molecular Weight
88,061 Da[Similar Products]
NCBI Official Full Name
FSH
NCBI Official Synonym Full Names
bromodomain containing 2
NCBI Official Symbol
BRD2??[Similar Products]
NCBI Official Synonym Symbols
FSH; NAT; RNF3; FSRG1; RING3; D6S113E; FLJ31942; KIAA9001; DKFZp686N0336; BRD2
??[Similar Products]
NCBI Protein Information
bromodomain-containing protein 2; O27.1.1; OTTHUMP00000178758; bromodomain-containing 2; really interesting new gene 3 protein; female sterile homeotic-related gene 1
UniProt Protein Name
Bromodomain-containing protein 2
UniProt Synonym Protein Names
O27.1.1; Really interesting new gene 3 protein
Protein Family
Bromodomain-containing protein
UniProt Gene Name
BRD2??[Similar Products]
UniProt Synonym Gene Names
KIAA9001; RING3??[Similar Products]
UniProt Entry Name
BRD2_HUMAN
NCBI Summary for BRD2
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
UniProt Comments for BRD2
BRD2: an atypical protein kinase with two bromodomains that bind to acetylated lysine residues. Specifically interacts with transcriptionally active chromatin. Elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. Interacts with E2F1 and with histone H4 acetylated at Lys-13. May play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Kinase, protein; ATYPICAL group; BRD family
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; chromatin binding
Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; transcription, DNA-dependent; spermatogenesis; chromatin modification
Product References and Citations for anti-BRD2 antibody
No references.
Research Articles on BRD2
1. Data report the crystal structures of the N-terminal bromodomain of human BRD2 (BRD2-BD1; residues 74-194) in complex with each of three different Lys-12-acetylated histone H4 peptides.
2. Studies indicate that KSHV LANA interacts with Brd2 and Brd4.
3. no difference was observed in the allele and genotype frequencies of BRD2 between juvenile myoclonic epilepsy (JME) and controls
4. The BRD2 gene is located within the human class II major histocompatibility complex at Chromosome 6p21.3, but appears to have no role in antigen processing, and is likely involved in signal transduction and transcription.
5. Brd2 is a novel protein kinase that plays a role in cell cycle-responsive transcription, recruiting E2F1, TATA Box Binding Protein and histone acetylase activity to E2F-responsive promoters in response to mitogenic stimuli.
6. Brd2 associates with the transcription factor E2F, the Mediator components CDK8 and TRAP220, RNA polymerase II on chromatin; and associates with acetylated histone H4 through its bromodomains.
7. The second bromodomain of Brd2 binds histone H4 acetyl-lysine K12.
8. The BRD2 gene is homologous to the mammalian cell cycle regulator TAFII250 and the Drosophila trithorax group gene female sterile homeotic; the gene encodes a nuclear-localized kinase with signal transduction activity.
9. Brd2-driven B cell lymphomas exhibit a transcriptional signature of diffuse large B cell lymphoma, and show differential expression of genes involved in normal proliferation, as well as several novel, tumor-specific genes.
10. Brd2, a nuclear-localized protein kinase, binds to cyclin A promoter chromatin and recruits histone H4-specific acetylase activity, increasing cyclin A transcription and accelerating S phase entry in fibroblasts.
11. B cell-restricted expression of Brd2 in transgenic mice upregulates cyclin A trancription even in mitogenically unstimulated B cells and causes lymphoma with a B1a-like immunophenotype, accompanied by peripheral B cell leukemia.
12. Results report that the double bromodomain proteins Brd2 and Brd3 associate preferentially in vivo with hyperacetylated chromatin along the entire lengths of transcribed genes.
13. Observational study of gene-disease association. (HuGE Navigator)
14. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
15. study investigated the SNP markers (rs188254, rs206781, and rs516535) in the 3'-end of the BRD2 and found no significant difference between the allele frequencies in epilepsy cases and controls
16. Brd2 bromodomain 2 is monomeric in solution and dynamically interacts with H4-AcK12; additional secondary elements in the long ZA loop may be a common characteristic of BET bromodomains.
17. Evidence from transgenic mice suggests that Brd2 transactivates the cyclin A locus, and that overexpression increases cyclin A transcription, destabilizing the cell cycle.
18. immunoblot and immunoprecipitation experiments to identify proteins interacting with Fsrg1 and RING3
19. Present results support evidence that photoparoxysmal response and Myoclonic Epilepsy, Juvenile share epileptogenic pathways, for which BRD2 might be an underlying susceptibility gene.
20. The results showed that BRD7 could interact with BRD2 and the region from amino acid 430 to 798 of BRD2 was critical for the interaction of BRD2 with BRD7. BRD2 mainly localizes in nucleus and BRD2 has distinct roles in initiating apoptosis.
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