Product Name
GABAA Receptor g2 (GABRG2), Polyclonal Antibody
Full Product Name
Anti-GABAA Receptor, gamma2-Subunit
Product Gene Name
anti-GABRG2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_005109.3 (26934991..27024440, complement). Location: 10q21
3D Structure
ModBase 3D Structure for P18508
Specificity
Specific for the ~46k gamma2-subunit of the GABAA receptor in Western blots of rat brain extracts.
Purity/Purification
Neat Serum (Unpurified neat serum.)
Form/Format
50 ul neat serum. Adequate material to conduct 10 mini-Western blots.
Antigen
Peptide representing a sequence that is specific for the gamma2-subunit of rat GABAA receptor. The sequence of the immunogen has no homology with any other GABAA R-subunit.
Immunogen Information
Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH
Reactivity Assumed Based on 100% Sequence Homology
Chicken, human, mouse, non-human primates and zebra fish
Species Reactivity Note
The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with bovine, chicken, human, mouse, non-human primate and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; P?ltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GABRG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GABRG2 antibody
Rabbit polyclonal antibody
Applications Tested/Suitable for anti-GABRG2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GABRG2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400
Testing Data of anti-GABRG2 antibody
NCBI/Uniprot data below describe general gene information for GABRG2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_899156.1
[Other Products]
NCBI GenBank Nucleotide #
NM_183327.1
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UniProt Primary Accession #
P18508
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UniProt Related Accession #
P18508[Other Products]
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit gamma-2
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, gamma 2
NCBI Official Symbol
Gabrg2??[Similar Products]
NCBI Protein Information
gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit gamma-2
UniProt Synonym Protein Names
GABA(A) receptor subunit gamma-2
Protein Family
Gamma-aminobutyric acid receptor
UniProt Gene Name
Gabrg2??[Similar Products]
UniProt Entry Name
GBRG2_RAT
NCBI Summary for GABRG2
subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]
UniProt Comments for GABRG2
Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
Subcellular location: Cell junction ? synapse ? postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Post-translational modification: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability
By similarity.
Miscellaneous: This subunit carries the benzodiazepine .
Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]
Product References and Citations for anti-GABRG2 antibody
??Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97
??McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
??Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
??Ogris W, P?ltl A, Hauer B, Ernst M, Oberto A, Wulff P, H?ger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
??Olsen RW, Tobin AJ (1990) Molecular
biology of GABAA receptors. FASEB 4:1469-1480.
Research Articles on GABRG2
1. Data indicate there was an inverse correlation between depressive-like behaviors and GABA(A)R alpha1 subunit mRNA expression in the right hemisphere in female and progesterone decreased the GABA(A)R gamma2 mRNA expression only in the left hemisphere of male.
Precautions
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