Product Name
GABAA Receptor, g 2 (Ser327) (GABRG2), Polyclonal Antibody
Popular Item
Full Product Name
Anti-Phospho-Ser327 GABAA Receptor, gamma2 subunit
Product Gene Name
anti-GABRG2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_005109.3 (26934991..27024440, complement). Location: 10q21
3D Structure
ModBase 3D Structure for P18508
Specificity
Specific for ~45k GABAA receptor gamma y2 subunit phosphorylated at Ser327 in Western Blots of rat brain homogenate.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of the GABAA receptor, gamma2 subunit.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, human and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCepsilon regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)
Preparation and Storage
For long term storage ~80 degree C is recommended, but shorter term storage at ~20 degree C is also acceptable as aliquots maybe taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.
Other Notes
Small volumes of anti-GABRG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GABRG2 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GABRG2 antibody
Dot Blots (DB), Western Blot (WB)
Application Notes for anti-GABRG2 antibody
Quality Control:
DB: 1:1000
WB: 1:1000
Quality Control Tests: Western Blots performed on each lot.
Testing Data of anti-GABRG2 antibody
NCBI/Uniprot data below describe general gene information for GABRG2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_899156.1
[Other Products]
NCBI GenBank Nucleotide #
NM_183327.1
[Other Products]
UniProt Primary Accession #
P18508
[Other Products]
UniProt Related Accession #
P18508[Other Products]
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit gamma-2
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, gamma 2
NCBI Official Symbol
Gabrg2??[Similar Products]
NCBI Protein Information
gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit gamma-2
UniProt Synonym Protein Names
GABA(A) receptor subunit gamma-2
Protein Family
Gamma-aminobutyric acid receptor
UniProt Gene Name
Gabrg2??[Similar Products]
UniProt Entry Name
GBRG2_RAT
NCBI Summary for GABRG2
subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]
UniProt Comments for GABRG2
Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
Subcellular location: Cell junction ? synapse ? postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Post-translational modification: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability
By similarity.
Miscellaneous: This subunit carries the benzodiazepine .
Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]
Product References and Citations for anti-GABRG2 antibody
??Olsen RW, Tobin AJ (1990) Molecular
biology of GABAA receptors. FASEB 4:1469-1480.
??Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653
??Ogris W, P?ltl A, Hauer B, Ernst M, Oberto A, Wulff P, H?ger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
??Qi ZH, Song M, Wallace MJ, Wang D, Newton PM, McMahon T, Chou WH, Zhang C, Shokat KM, Messing RO (2007) Protein kinase Cepsilon regulates gamma-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of gamma2 subunits. J Biol Chem 282(45):33052-63.
Research Articles on GABRG2
1. Data indicate there was an inverse correlation between depressive-like behaviors and GABA(A)R alpha1 subunit mRNA expression in the right hemisphere in female and progesterone decreased the GABA(A)R gamma2 mRNA expression only in the left hemisphere of male.
Precautions
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