Full Product Name
Anti-FGF23 Antibody
Product Synonym Names
Fibroblast growth factor 23; FGF-23; Fgf23
Product Gene Name
anti-FGF23 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8VI82
Species Reactivity
Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Immunogen
E Coli-derived rat FGF23 recombinant protein (Position: Y25-V251).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-FGF23 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FGF23 antibody
Description: Rabbit IgG polyclonal antibody for FGF23 detection. Tested with WB, Direct ELISA in Mouse; Rat.
Background: Fibroblast growth factor 23?or?FGF23?is a?protein?that in humans is encoded by the?FGF23?gene. This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of
biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC).
Applications Tested/Suitable for anti-FGF23 antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes for anti-FGF23 antibody
WB: 0.1-0.5 mug/ml
Direct ELISA: 0.1-0.5 mug/ml
Tested Species: In-house tested species with positive results.
Western Blot (WB) of anti-FGF23 antibody
Figure 1. Western blot analysis of FGF23 using anti-FGF23 antibody.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
NCBI/Uniprot data below describe general gene information for FGF23. It may not necessarily be applicable to this product.
NCBI Accession #
NP_570110.1
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NCBI GenBank Nucleotide #
NM_130754.1
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UniProt Primary Accession #
Q8VI82
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UniProt Related Accession #
Q8VI82[Other Products]
Molecular Weight
27,912 Da
NCBI Official Full Name
fibroblast growth factor 23
NCBI Official Synonym Full Names
fibroblast growth factor 23
NCBI Official Symbol
Fgf23??[Similar Products]
NCBI Protein Information
fibroblast growth factor 23
UniProt Protein Name
Fibroblast growth factor 23
Protein Family
Fibroblast growth factor
UniProt Gene Name
Fgf23??[Similar Products]
UniProt Synonym Gene Names
FGF-23??[Similar Products]
UniProt Comments for FGF23
Regulator of phosphate homeostasis (). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (). Regulator of vitamin-D metabolism (). Negatively regulates osteoblasts differentiation and matrix mineralization (). Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL.
Product References and Citations for anti-FGF23 antibody
1. "Entrez Gene: FGF23 fibroblast growth factor 23". 2. Fukumoto S (2008). "Physiological regulation and disorders of phosphate metabolism--pivotal role of fibroblast growth factor 23".?Intern. Med.?47? (5): 337-43. 3. Yamashita T, Yoshioka M, Itoh N (October 2000). "Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain".?Biochem. Biophys. Res. Commun.277? (2): 494-8.
Research Articles on FGF23
1. In chronic kidney disease (CKD) rat models, FGF23 mRNA is expressed in the kidney, and the FGF23 protein is expressed at high levels in osteopontin-positive renal tubule epithelium cells likely via TGFbeta1 stimulation. FGF23 produced in the kidney might contribute to P metabolism in subjects with CKD.
Precautions
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