Product Name
Sarcoglycan Alpha (SGCa), Recombinant Protein
Full Product Name
Recombinant Sarcoglycan Alpha (SGCa)
Product Synonym Names
SGC-A; A2; 50-DAG; ADL; DAG2; DMDA2; LGMD2D; SCARMD1; Adhalin; Dystroglycan-2; 50kDa Dystrophin-Associated Glycoprotein
Product Gene Name
SGCa recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q16586
Purity/Purification
> 90%
Form/Format
Freeze-dried powder
Tag
Two N-terminal Tags, His-tag and GST-tag
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Source Note
Prokaryotic expression
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Usage
Reconstitute in PBS or others.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of SGCa recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for SGCa recombinant protein
Recombinant Protein
Applications Tested/Suitable for SGCa recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
SDS-Page of SGCa recombinant protein
NCBI/Uniprot data below describe general gene information for SGCa. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000014.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000023.3
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UniProt Primary Accession #
Q16586
[Other Products]
UniProt Secondary Accession #
Q13710; Q13712; A6NEB8; A8K3K7[Other Products]
UniProt Related Accession #
Q16586[Other Products]
NCBI Official Full Name
alpha-sarcoglycan isoform 1
NCBI Official Synonym Full Names
sarcoglycan alpha
NCBI Official Symbol
SGCA??[Similar Products]
NCBI Official Synonym Symbols
ADL; DAG2; 50DAG; DMDA2; LGMD2D; SCARMD1; adhalin
??[Similar Products]
NCBI Protein Information
alpha-sarcoglycan
UniProt Protein Name
Alpha-sarcoglycan
UniProt Synonym Protein Names
50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Protein Family
Alpha-sarcoglycan
UniProt Gene Name
SGCA??[Similar Products]
UniProt Synonym Gene Names
ADL; DAG2; Alpha-SG; 50DAG??[Similar Products]
NCBI Summary for SGCa
This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Comments for SGCa
SGCA: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCA are the cause of limb-girdle muscular dystrophy type 2D (LGMD2D); also known as Duchenne- like muscular dystrophy autosomal recessive type 2 or severe childhood autosomal recessive muscular dystrophy (SCARMD). LGMD2D is an autosomal recessive degenerative myopathy characterized by progressive muscle wasting from early childhood with loss of independent ambulation by teenage years. Muscle biopsy shows necrosis, decreased immunostaining for alpha sarcoglycan, and adhalin deficiency. The phenotype is less severe than LGMD2C. Belongs to the sarcoglycan alpha/epsilon family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: dystrophin-associated glycoprotein complex; sarcoglycan complex
Molecular Function: protein binding
Biological Process: muscle contraction; muscle development
Disease: Muscular Dystrophy, Limb-girdle, Type 2d
Research Articles on SGCa
1. T in SGCA may be high in Taiwan, especially in the aboriginal population regardless of the tribes.">The results suggest that the carrier rate of c.101G>T in SGCA may be high in Taiwan, especially in the aboriginal population regardless of the tribes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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