For Research Use Only. Not for use in diagnostic procedures.

Store at 2 to 8 degree C for 6 months.

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Small volumes of SGLT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS164261 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/Glucose Cotransporter 2 (SGLT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SGLT2. The ELISA analytical biochemical technique of the MBS164261 kit is based on SGLT2 antibody-SGLT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SGLT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SGLT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Sodium, Glucose Cotransporter 2 (also known as SGLT2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SGLT2 antibody. SGLT2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SGLT2 Antibody is added and binds to SGLT2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SGLT2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SGLT2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

49,357 Da

solute carrier family 5 member 2

sodium/glucose cotransporter 2

Sodium/glucose cotransporter 2

; Na(+)/glucose cotransporter 2??[Similar Products]

This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene. [provided by RefSeq, Feb 2015]

Sodium-dependent glucose transporter. Has a Na+ to glucose coupling ratio of 1:1.

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