AMP; APRTase

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 97%

Freeze-dried powder

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.

Small volumes of APRT active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Enzyme & Kinase; Metabolic pathway; Kidney biomarker

Cell?culture, Activity?Assays

Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner.

Predicted Molecular Weight: 23.3kDa
Accurate Molecular Weight: 23kDa

adenine phosphoribosyltransferase

adenine phosphoribosyltransferase

Adenine phosphoribosyltransferase

APRT??[Similar Products]

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Protein type: EC 2.4.2.7; Nucleotide Metabolism - purine; Transferase

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: cytoplasm; cytosol; extracellular region; nucleoplasm

Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding

Biological Process: adenine salvage; cellular response to insulin stimulus; grooming behavior; lactation; neutrophil degranulation; purine ribonucleoside salvage; purine-containing compound salvage

Disease: Adenine Phosphoribosyltransferase Deficiency

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