Product Name
Adenine phosphoribosyltransferase (APRT), Recombinant Protein
Full Product Name
Recombinant Human Adenine phosphoribosyltransferase (APRT)
Product Synonym Names
Adenine phosphoribosyltransferase; APRT; EC=2.4.2.7
Product Gene Name
APRT recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-180aa; Full Length
Sequence
ADSELQLVEQ RIRSFPDFPT PGVVFRDISP VLKDPASFRA AIGLLARHLK ATHGGRIDYI AGLDSRGFLF GPSLAQELGL GCVLIRKRGK LPGPTLWASY SLEYGKAELE IQKDALEPGQ RVVVVDDLLA TGGTMNAACE LLGRLQAEVL ECVSLVELTS LKGREKLAPV PFFSLLQYE
3D Structure
ModBase 3D Structure for P07741
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>=90% (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Homo sapiens (Human)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of APRT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
APRT recombinant protein
Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Product Categories/Family for APRT recombinant protein
Signal Transduction
SDS-PAGE of APRT recombinant protein
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
NCBI/Uniprot data below describe general gene information for APRT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000476.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000485.2
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UniProt Primary Accession #
P07741
[Other Products]
UniProt Secondary Accession #
Q3KP55; Q68DF9; G5E9J2[Other Products]
UniProt Related Accession #
P07741[Other Products]
Molecular Weight
14,557 Da
NCBI Official Full Name
adenine phosphoribosyltransferase isoform a
NCBI Official Synonym Full Names
adenine phosphoribosyltransferase
NCBI Official Symbol
APRT??[Similar Products]
NCBI Official Synonym Symbols
AMP; APRTD
??[Similar Products]
NCBI Protein Information
adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase
UniProt Protein Name
Adenine phosphoribosyltransferase
Protein Family
Adenine phosphoribosyltransferase
UniProt Gene Name
APRT??[Similar Products]
UniProt Synonym Gene Names
APRT??[Similar Products]
UniProt Entry Name
APT_HUMAN
NCBI Summary for APRT
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for APRT
APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: Nucleotide Metabolism - purine; EC 2.4.2.7; Transferase
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding
Biological Process: lactation; grooming behavior; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine base metabolic process; purine salvage
Disease: Adenine Phosphoribosyltransferase Deficiency
Research Articles on APRT
1. Case study of 2 year old Japanese boy with APRT deficiency. Genetic analysis showed compound heterozygote APRT*J and missense mutation L33P. APRT deficiency should be suspected in patients with radiolucent kidney stones and urinary 2,8-DHA crystals.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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