Full Product Name
APRT Antibody
Product Synonym Names
AMP; APRTD
Product Gene Name
anti-APRT antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07741
Specificity
The antibody detects endogenous level of total APRT protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human APRT.
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-APRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APRT antibody
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-APRT antibody
Total protein Ab
Applications Tested/Suitable for anti-APRT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-APRT antibody
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Western Blot (WB) of anti-APRT antibody
Western blot analysis of extracts of various cell lines, using APRT antibody.
Immunohistochemistry (IHC) of anti-APRT antibody
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using APRT antibody at dilution of 1:200 (400x lens).
Immunofluorescence (IF) of anti-APRT antibody
Immunofluorescence analysis of A549 cell using APRT antibody.
NCBI/Uniprot data below describe general gene information for APRT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000476.1
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NCBI GenBank Nucleotide #
NM_000485.2
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UniProt Primary Accession #
P07741
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UniProt Secondary Accession #
Q3KP55; Q68DF9; G5E9J2[Other Products]
UniProt Related Accession #
P07741[Other Products]
Molecular Weight
14,557 Da
NCBI Official Full Name
adenine phosphoribosyltransferase isoform a
NCBI Official Synonym Full Names
adenine phosphoribosyltransferase
NCBI Official Symbol
APRT??[Similar Products]
NCBI Official Synonym Symbols
AMP; APRTD
??[Similar Products]
NCBI Protein Information
adenine phosphoribosyltransferase
UniProt Protein Name
Adenine phosphoribosyltransferase
Protein Family
Probable adenine phosphoribosyltransferase
UniProt Gene Name
APRT??[Similar Products]
UniProt Synonym Gene Names
APRT??[Similar Products]
UniProt Entry Name
APT_HUMAN
NCBI Summary for APRT
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for APRT
APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: Transferase; Nucleotide Metabolism - purine; EC 2.4.2.7
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding
Biological Process: grooming behavior; lactation; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine base metabolic process; purine salvage
Disease: Adenine Phosphoribosyltransferase Deficiency
Research Articles on APRT
1. A new mutation, p.Gln147X, in APRT gene, was found in a patient with adenine phosphoribosyltransferase deficiency.
Precautions
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Disclaimer
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