Endoribonuclease Dicer; DCR; DCR1; Dcr1 homolog (Drosophila); Dicer 1; Dicer 1 ribonuclease type III; Dicer; DICER_HUMAN; Dicer1; Double-strand-specific ribonuclease; Endoribonuclease Dicer; Helicase moi; Helicase with RNAse motif; HERNA; KIAA0928; MNG1; RMSE2; dicer 1, ribonuclease type III

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Immunogen Affinity Purified

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of anti-DICER1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Description: Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.

Background: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Anti- Dicer antibody, Western blotting
All lanes: Anti Dicer at 0.5ug/ml
WB: Recombinant Human Dicer Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD

Anti- Dicer antibody, Western blotting
All lanes: Anti Dicer at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 217KD
Observed bind size: 217KD

Anti- Dicer antibody, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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DICER_HUMAN

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: Ribonuclease; EC 3.1.26.3; RNA-binding; Helicase; RNA processing; Cell development/differentiation

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: axon; cytoplasm; cytosol; dendrite; ER-Golgi intermediate compartment; growth cone; nucleus

Molecular Function: ATP binding; deoxyribonuclease I activity; double-stranded RNA binding; endoribonuclease activity; helicase activity; metal ion binding; protein binding; protein domain specific binding; ribonuclease III activity; siRNA binding

Biological Process: DNA fragmentation during apoptosis; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; negative regulation of transcription from RNA polymerase II promoter; nerve development; neurite morphogenesis; positive regulation of myelination; positive regulation of Schwann cell differentiation; pre-microRNA processing; RNA interference, conversion of ds siRNA to ss siRNA; RNA interference, production of siRNA; RNA interference, siRNA loading onto RISC; RNA interference, targeting of mRNA for destruction; RNA-mediated gene silencing

Disease: Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Pleuropulmonary Blastoma; Rhabdomyosarcoma, Embryonal, 2

1. Hutvagner, G., McLachlan, J., Pasquinelli, A. E., Balint, E., Tuschl, T., Zamore, P. D. A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838, 2001. 2. Matsuda, S., Ichigotani, Y., Okuda, T., Irimura, T., Nakatsugawa, S., Hamaguchi, M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta 1490: 163-169, 2000.

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