Dicer; HERNA; Dicer 1,Double-Stranded RNA-Specific Endoribonuclease; Helicase with RNase motif; Helicase MOI

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Small volumes of DICER1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).

92,726 Da

dicer 1 ribonuclease III

endoribonuclease Dicer

Endoribonuclease Dicer

DICER; HERNA; KIAA0928; Helicase MOI??[Similar Products]

DICER_HUMAN

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: Cell development/differentiation; Helicase; EC 3.1.26.3; Ribonuclease; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: axon; cytoplasm; cytosol; dendrite; ER-Golgi intermediate compartment; growth cone; nucleus

Molecular Function: ATP binding; deoxyribonuclease I activity; DNA binding; double-stranded RNA binding; endoribonuclease activity; helicase activity; metal ion binding; miRNA binding; protein binding; protein domain specific binding; ribonuclease III activity; siRNA binding

Biological Process: angiogenesis; branching morphogenesis of a tube; cardiac muscle cell development; cartilage development; cerebral cortex development; defense response to virus; DNA fragmentation during apoptosis; embryonic hindlimb morphogenesis; gene expression; hair follicle morphogenesis; inner ear receptor cell development; lung development; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; mRNA stabilization; multicellular organism growth; myelin formation in the peripheral nervous system; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of transcription from RNA polymerase II promoter; nerve development; neurite morphogenesis; olfactory bulb interneuron differentiation; positive regulation of myelination; positive regulation of Schwann cell differentiation; post-embryonic development; pre-microRNA processing; regulation of cell cycle; regulation of neuron differentiation; regulation of oligodendrocyte differentiation; regulation of viral genome replication; reproductive structure development; RNA interference, conversion of ds siRNA to ss siRNA; RNA interference, production of siRNA; RNA interference, siRNA loading onto RISC; RNA interference, targeting of mRNA for destruction; RNA-mediated gene silencing; spinal cord motor neuron differentiation; spindle assembly; spleen development; stem cell maintenance; zygote asymmetric cell division

Disease: Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Pleuropulmonary Blastoma; Rhabdomyosarcoma, Embryonal, 2

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