Adenine phosphoribosyltransferase; AMP; Adenine phosphoribosyltransferase AMP diphosphorylase; AMP pyrophosphorylase; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase.

For Research Use Only. Not for use in diagnostic procedures.

E Coli

> 90% by SDS-PAGE

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay) (lot specific)

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of APRT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E Coli and purified by using conventional chromatography techniques.

Metabolism

SDS-PAGE

19.6 kDa (180aa), confirmed by MALDI-TOF.

adenine phosphoribosyltransferase

adenine phosphoribosyltransferase

Adenine phosphoribosyltransferase

APRT??[Similar Products]

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Baranowska-Bosiacka I., et al. (2009) Toxicology. 259 (1-2):77-83.; Liang L., et al. (2007) Cancer Res. 67 (5):1910-7.;

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