Full Product Name
Anti-APRT Antibody (aa149-179) IHC-plus
Product Synonym Names
Rabbit Polyclonal to Human APRT; Human APRT; AMP; AMP diphosphorylase; APRTD; AMP pyrophosphorylase; Transphosphoribosidase
Product Gene Name
anti-APRT antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07741
Purity/Purification
Immunoaffinity purified
Form/Format
PBS with 0.09% (w/v) sodium azide
Immunogen
APRT antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human APRT.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human APRT.
Immunogen Type
Synthetic peptide
Antigen Modification
aa149-179
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Other Notes
Small volumes of anti-APRT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-APRT antibody
APRT Antibody, AMP Antibody, AMP diphosphorylase Antibody, APRTD Antibody, AMP pyrophosphorylase Antibody, Transphosphoribosidase Antibody Description: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
Applications Tested/Suitable for anti-APRT antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-APRT antibody
IHC-P (5 ug/ml)
WB (1:1000)
Immunohistochemistry (IHC) of anti-APRT antibody
Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)
Western Blot (WB) of anti-APRT antibody
APRT Antibody western blot of MCF-7 cell line and rat kidney tissue lysates (35 ug/lane). The APRT antibody detected the APRT protein (arrow).
NCBI/Uniprot data below describe general gene information for APRT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000476.1
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NCBI GenBank Nucleotide #
NM_000485.2
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UniProt Primary Accession #
P07741
[Other Products]
UniProt Secondary Accession #
Q3KP55; Q68DF9; G5E9J2[Other Products]
UniProt Related Accession #
P07741[Other Products]
Molecular Weight
14,557 Da
NCBI Official Full Name
adenine phosphoribosyltransferase isoform a
NCBI Official Synonym Full Names
adenine phosphoribosyltransferase
NCBI Official Symbol
APRT??[Similar Products]
NCBI Official Synonym Symbols
AMP; APRTD
??[Similar Products]
NCBI Protein Information
adenine phosphoribosyltransferase
UniProt Protein Name
Adenine phosphoribosyltransferase
Protein Family
Probable adenine phosphoribosyltransferase
UniProt Gene Name
APRT??[Similar Products]
UniProt Synonym Gene Names
APRT??[Similar Products]
UniProt Entry Name
APT_HUMAN
NCBI Summary for APRT
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for APRT
APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: EC 2.4.2.7; Transferase; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: cytoplasm; cytosol; nucleoplasm
Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding
Biological Process: adenine salvage; cellular response to insulin stimulus; grooming behavior; lactation; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; purine salvage
Disease: Adenine Phosphoribosyltransferase Deficiency
Research Articles on APRT
1. A new mutation, p.Gln147X, in APRT gene, was found in a patient with adenine phosphoribosyltransferase deficiency.
Precautions
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