CBFB; core-binding factor, beta subunit; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; core-binding factor subunit beta; polyomavirus enhancer; CBFB antibody; core-binding factor; beta subunit antibody; PEBP2B antibody; CBF-beta antibody; PEA2-beta antibody; PEBP2-beta antibody; SL3-3 enhancer factor 1 beta subunit antibody; SL3-3 enhancer factor 1 subunit beta antibody; SL3/AKV core-binding factor beta subunit antibody; core-binding factor subunit beta antibody; polyomavirus enhancer binding protein 2; beta subunit antibody; polyomavirus enhancer-binding protein 2 beta subunit antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-CBFB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 22kDa band observed in lysates of cell line Daudi (calculated MW of 22. 0kDa according to NP_074036.1). Recommended concentration: 0.3-1ug/ml.

(0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

21,991 Da

core-binding factor, beta subunit

core-binding factor subunit beta

Core-binding factor subunit beta

CBF-beta; PEA2-beta; PEBP2-beta??[Similar Products]

PEBB_HUMAN

The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CBFB: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. Belongs to the CBF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: membrane; nucleus

Molecular Function: DNA binding; protein binding; transcription coactivator activity; transcription factor activity

Biological Process: cell maturation; lymphocyte differentiation; myeloid cell differentiation; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; protein polyubiquitination; transcription from RNA polymerase II promoter

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