Full Product Name
CBFB siRNA (Human)
Product Synonym Names
Core-binding factor subunit beta; CBF-beta; Polyomavirus enhancer-binding protein 2 beta subunit; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit
Product Gene Name
CBFB sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13951
Specificity
CBFB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CBFB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CBFB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CBFB sirna
siRNA to inhibit CBFB expression using RNA interference
Applications Tested/Suitable for CBFB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CBFB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001746.1
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NCBI GenBank Nucleotide #
NM_001755.2
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UniProt Primary Accession #
Q13951
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UniProt Secondary Accession #
Q13124; Q9HCT2; A8K347[Other Products]
UniProt Related Accession #
Q13951[Other Products]
Molecular Weight
21,991 Da
NCBI Official Full Name
core-binding factor subunit beta isoform 2
NCBI Official Synonym Full Names
core-binding factor, beta subunit
NCBI Official Symbol
CBFB??[Similar Products]
NCBI Official Synonym Symbols
PEBP2B
??[Similar Products]
NCBI Protein Information
core-binding factor subunit beta
UniProt Protein Name
Core-binding factor subunit beta
UniProt Synonym Protein Names
Polyomavirus enhancer-binding protein 2 beta subunit; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit
Protein Family
Core-binding factor
UniProt Gene Name
CBFB??[Similar Products]
UniProt Synonym Gene Names
CBF-beta; PEA2-beta; PEBP2-beta??[Similar Products]
UniProt Entry Name
PEBB_HUMAN
NCBI Summary for CBFB
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CBFB
CBFB: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM- CSF promoters. CBFB enhances DNA binding by RUNX1. A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. Belongs to the CBF-beta family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: membrane; nucleus
Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; osteoblast differentiation; lymphocyte differentiation; cell maturation; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation
Research Articles on CBFB
1. CBF-beta promoted steady-state levels of HIV-1 Vif by inhibiting the degradation of HIV-1 Vif through the proteasome pathway.
Precautions
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Disclaimer
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