For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_005116.2 (88832905..89078870). Location: 17q12.3

Monoclonal

IgG1

S8b-1

Mouse

This antibody recognizes human, mouse, and rat Cavbeta2. It does not cross-react with Cav beta1, Cav beta3, or Cav beta4.

1.0mg/ml in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.

Small volumes of anti-Cacnb2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Ion channels like anti Cav beta 2 calcium channel - CACNB - are localized to cell plasma membrane and activation with ligands results in cellular responses. With limited information about molecular subunit composition for active voltage dependent calcium channels, MyBiosource's mouse monoclonal antibody recognized beta specifically and not gamma or alpha. Cell experiments to determine functional properties commonly utilize stable transfections of human or mouse gene plasmid lenti activation system expression or ShRNA plasmid sc knockdown. The ability to distinguish subtypes of expression in either stably puromycin type selection or stable gene silencing puromyocin selection requires our pan species [ human mouse rat ] CACNB2 antibody. Compared to rabbit antibodies, our Cav B2 calcium channel voltage antibody recognizes protein in lysates, IHC and is suitable for ELISA kits development. Value pricing, rapid shipping and promotional items make this product and all our products top sellers worldwide. Find us at MyBiosource.com.

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Cavbeta subunits are involved in the transport of the pore-forming alpha1 subunit to the plasma membrane. They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the poreforming subunit to the target membrane.

Immunoblot (IB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunofluorescence (IF)

Immunoblotting: use at 1-10ug/ml.
Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml.
Immunofluorescence: use at 1-10ug/ml.

These are recommended concentrations.
User should determine optimal concentrations for their application.
Positive control: Rat brain lysate

Adult rat brain lysate.
A band of ~78kDa is detected.

calcium channel, voltage-dependent, beta 2 subunit

voltage-dependent L-type calcium channel subunit beta-2; CAB2; calcium channel voltage-dependent subunit beta 2

Voltage-dependent L-type calcium channel subunit beta-2

Cacnlb2??[Similar Products]

CACB2_RAT

auxillary subunit of the L-type Ca(2+) channel [RGD]

CACNB2: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRGDA4). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel beta subunit family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, calcium

Cellular Component: voltage-gated calcium channel complex; sarcolemma

Molecular Function: voltage-gated calcium channel activity; protein domain specific binding; calcium channel regulator activity; high voltage-gated calcium channel activity; phosphoprotein binding; protein kinase binding

Biological Process: synaptic transmission; visual perception; calcium ion transport; positive regulation of calcium ion transport; neuromuscular junction development

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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