Product Name
CavBeta2, Monoclonal Antibody
Full Product Name
CavBeta2 Antibody, Clone S8B-1: Dylight 488
Product Synonym Names
Mouse Anti-Rat Cav beta 2 Monoclonal IgG1; Cav beta 2 Antibody: Dylight 488; Cacnlb2 Antibody; cacnb2 Antibody; Voltage-dependent L-type calcium channel subunit beta-2 Antibody; CAB2 Antibody
Product Gene Name
anti-CavBeta2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8VGC3
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~78 kDa. No cross reactivity against Cavbeta1, Cavbeta3, Cavbeta4.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide amino acids 189-205 of rat CavBeta2
Cellular Localization
Cell Membrane; Sarcolemma
Certificate of Analysis
1 ug/ml was sufficient for detection of Cavbeta2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, F?rster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-CavBeta2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CavBeta2 antibody
Cav Beat subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane (1). They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore-forming subunit to the target membrane (2, 3). They also determine the biophysical properties of the calcium channel (3).
Product Categories/Family for anti-CavBeta2 antibody
Neuroscience; Ion Channels; Calcium Channels; Voltage-Gated Calcium Channels
Applications Tested/Suitable for anti-CavBeta2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Antibody Microarray (AM)
Application Notes for anti-CavBeta2 antibody
WB: 1:1000
IHC: 1:1000
ICC/IF: 1:100
Immunohistochemistry (IHC) of anti-CavBeta2 antibody
Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.
Western Blot (WB) of anti-CavBeta2 antibody
Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry (ICC) of anti-CavBeta2 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.
NCBI/Uniprot data below describe general gene information for CavBeta2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446303.1
[Other Products]
NCBI GenBank Nucleotide #
NP_446303.1
[Other Products]
UniProt Primary Accession #
Q8VGC3
[Other Products]
UniProt Related Accession #
Q8VGC3[Other Products]
NCBI Official Full Name
voltage-dependent L-type calcium channel subunit beta-2
NCBI Official Synonym Full Names
calcium voltage-gated channel auxiliary subunit beta 2
NCBI Official Symbol
Cacnb2??[Similar Products]
NCBI Official Synonym Symbols
Cacnlb2
??[Similar Products]
NCBI Protein Information
voltage-dependent L-type calcium channel subunit beta-2
UniProt Protein Name
Voltage-dependent L-type calcium channel subunit beta-2
UniProt Synonym Protein Names
Calcium channel voltage-dependent subunit beta 2
UniProt Gene Name
Cacnb2??[Similar Products]
UniProt Synonym Gene Names
Cacnlb2??[Similar Products]
UniProt Entry Name
CACB2_RAT
NCBI Summary for CavBeta2
auxillary subunit of the L-type Ca(2+) channel [RGD, Feb 2006]
UniProt Comments for CavBeta2
CACNB2: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRGDA4). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel beta subunit family. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, calcium
Cellular Component: voltage-gated calcium channel complex; sarcolemma
Molecular Function: voltage-gated calcium channel activity; protein domain specific binding; calcium channel regulator activity; high voltage-gated calcium channel activity; phosphoprotein binding; protein kinase binding
Biological Process: synaptic transmission; visual perception; calcium ion transport; positive regulation of calcium ion transport; neuromuscular junction development
Product References and Citations for anti-CavBeta2 antibody
1. Dolphin A.C. (2003) J Bioenerg. Biomembr. 35: 599-620. 2. Bichet D., et al. (2000) Neuron. 25: 177-190. 3. Xie M., et al. (2007) J Cell Biol. 178(3): 489-502.
Research Articles on CavBeta2
1. these findings provide evidence for a new role of Homer1 supporting the regulation of Cav1.2 channels by STIM1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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