Product Name
72 kDa type IV collagenase (MMP2), ELISA Kit
Full Product Name
Human 72 kDa type IV collagenase ELISA Kit
Product Synonym Names
72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1; MMP2; CLG4A; 3.4.24.24
Product Gene Name
MMP2 elisa kit
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P08253
Detection Range
0.312-20 ng/mL
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of MMP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MMP2 purchase
MBS2884875 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 72 kDa type IV collagenase (MMP2) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP2. The ELISA analytical biochemical technique of the MBS2884875 kit is based on MMP2 antibody-MMP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
NCBI/Uniprot data below describe general gene information for MMP2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001121363.1
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NCBI GenBank Nucleotide #
NM_001127891.2
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UniProt Primary Accession #
P08253
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UniProt Secondary Accession #
Q9UCJ8; B2R6U1; B4DWH3; E9PE45[Other Products]
UniProt Related Accession #
P08253[Other Products]
Molecular Weight
68,831 Da
NCBI Official Full Name
72 kDa type IV collagenase isoform 2
NCBI Official Synonym Full Names
matrix metallopeptidase 2
NCBI Official Symbol
MMP2??[Similar Products]
NCBI Official Synonym Symbols
CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
??[Similar Products]
NCBI Protein Information
72 kDa type IV collagenase
UniProt Protein Name
72 kDa type IV collagenase
UniProt Synonym Protein Names
72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2
Protein Family
72 kDa type IV collagenase
UniProt Gene Name
MMP2??[Similar Products]
UniProt Synonym Gene Names
CLG4A; MMP-2??[Similar Products]
UniProt Entry Name
MMP2_HUMAN
NCBI Summary for MMP2
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
UniProt Comments for MMP2
MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.
Protein type: Cell development/differentiation; Secreted, signal peptide; Protease; Apoptosis; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.24
Chromosomal Location of Human Ortholog: 16q12.2
Cellular Component: extracellular region; extracellular space; mitochondrion; nucleus; plasma membrane; proteinaceous extracellular matrix; sarcomere
Molecular Function: metalloendopeptidase activity; metallopeptidase activity; protein binding; serine-type endopeptidase activity; zinc ion binding
Biological Process: angiogenesis; axon guidance; blood vessel maturation; cellular protein metabolic process; collagen catabolic process; embryo implantation; ephrin receptor signaling pathway; extracellular matrix disassembly; extracellular matrix organization and biogenesis; intramembranous ossification; positive regulation of innate immune response; proteolysis; response to hypoxia
Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy
Research Articles on MMP2
1. MMP-2 is a direct target of miR-519d-3p.
Precautions
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