72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; Mmp2; 3.4.24.24

For Research Use Only. Not for use in diagnostic procedures.

For long term storage, please store the entire kit at -20 degree C.

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Small volumes of Mmp2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS289590 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 72 kDa type IV collagenase (Mmp2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Mmp2. The ELISA analytical biochemical technique of the MBS289590 kit is based on Mmp2 antibody-Mmp2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Mmp2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Mmp2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

65,883 Da

matrix metallopeptidase 2

72 kDa type IV collagenase

72 kDa type IV collagenase

MMP-2??[Similar Products]

MMP2_MOUSE

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016]

MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.

Protein type: Cell development/differentiation; Apoptosis; Secreted, signal peptide; Motility/polarity/chemotaxis; EC 3.4.24.24; Secreted; Protease

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; membrane; mitochondrion; nucleus; plasma membrane; proteinaceous extracellular matrix; sarcomere

Molecular Function: fibronectin binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Biological Process: angiogenesis; axon regeneration in the peripheral nervous system; blood vessel maturation; cell migration; collagen catabolic process; embryo implantation; heart development; intramembranous ossification; negative regulation of cell adhesion; negative regulation of vasoconstriction; ovarian follicle development; positive regulation of apoptosis; positive regulation of cell migration; positive regulation of innate immune response; proteolysis; response to drug; response to estrogen stimulus; response to hydrogen peroxide; response to hypoxia; response to mechanical stimulus; response to oxidative stress; skeletal morphogenesis; tissue remodeling

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