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72 kDa type IV collagenase, Recombinant Protein

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產(chǎn)品名稱: 72 kDa type IV collagenase, Recombinant Protein
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72 kDa type IV collagenase, Recombinant Protein


72 kDa type IV collagenase, Recombinant Protein  的詳細(xì)介紹
Product Name

72 kDa type IV collagenase (Mmp2), Recombinant Protein

Full Product Name

Recombinant Rat 72 kDa type IV collagenase

Product Synonym Names
72 kDa gelatinaseGelatinase A; Matrix metalloproteinase-2; MMP-2
Product Gene Name

Mmp2 recombinant protein

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sequence Positions
110-662aa; Full Length
Sequence
YNFFPRKPKW DKNQITYRII GYTPDLDPET VDDAFARALK VWSDVTPLRF SRIHDGEADI MINFGRWEHG DGYPFDGKDG LLAHAFAPGT GVGGDSHFDD DELWTLGEGQ VVRVKYGNAD GEYCKFPFLF NGREYSSCTD TGRSDGFLWC STTYNFEKDG KYGFCPHEAL FTMGGNGDGQ PCKFPFRFQG TSYNSCTTEG RTDGYRWCGT TEDYDRDKKY GFCPETAMST VGGNSEGAPC VFPFTFLGNK YESCTSAGRS DGKVWCATTT NYDDDRKWGF CPDQGYSLFL VAAHEFGHAM GLEHSQDPGA LMAPIYTYTK NFRLSNDDIK GIQELYGPSP DADTDTGTGP TPTLGPVTPE ICKQDIVFDG IAQIRGEIFF FKDRFIWRTV TPRDKPTGPL LVATFWPELP EKIDAVYEAP QEEKAVFFAG NEYWVYSAST LERGYPKPLT SLGLPPDVQQ VDAAFNWSKN KKTYIFSGDK FWRYNEVKKK MDPGFPKLIA DSWNAIPDNL DAVVDLQGGG HSYFFKGAYY LKLENQSLKS VKFGSIKSDW LGC
3D Structure
ModBase 3D Structure for P33436
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater than 90% as determined by SDS-PAGE. (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Mmp2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
Mmp2 recombinant protein
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta3 on the surface of blood vessels.

SDS-PAGE of Mmp2 recombinant protein
Mmp2 recombinant protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
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NCBI/Uniprot data below describe general gene information for Mmp2. It may not necessarily be applicable to this product.
NCBI GI #
146262019
NCBI GeneID
81686
NCBI Accession #
NP_112316.2 [Other Products]
NCBI GenBank Nucleotide #
NM_031054.2 [Other Products]
UniProt Primary Accession #
P33436 [Other Products]
UniProt Secondary Accession #
P97581; Q6GMM9[Other Products]
Molecular Weight
78.1kD
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NCBI Official Full Name
72 kDa type IV collagenase
NCBI Official Synonym Full Names
matrix metallopeptidase 2
NCBI Official Symbol
Mmp2??[Similar Products]
UniProt Protein Name
72 kDa type IV collagenase
UniProt Synonym Protein Names
72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2
Protein Family
72 kDa type IV collagenase
UniProt Gene Name
Mmp2??[Similar Products]
UniProt Synonym Gene Names
MMP-2??[Similar Products]
UniProt Entry Name
MMP2_RAT
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NCBI Summary for Mmp2
plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]
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UniProt Comments for Mmp2
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().
Product References and Citations for Mmp2 recombinant protein
Homology cloning of rat 72 kDa type IV collagenase cytokine and second-messenger inducibility in glomerular mesangial cells.Marti H.P., McNeil L., Davies M., Martin J., Lovett D.H.Biochem. J. 291:441-446(1993) The cloning of the cDNA encoding rat gelatinase A from a rat skin wound cDNA library.Okada A., Basset P.

Research Articles on Mmp2
1. Angiotensin II regulates MMP-2, MMP-9, and TIMP-1 expression and affects collagen deposition.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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