Product Name
SHFM1, siRNA
Full Product Name
SHFM1 siRNA (Human)
Product Synonym Names
DSS1; SHFDG1; 26S proteasome complex subunit DSS1; Deleted in split hand/split foot protein 1; Split hand/foot deleted protein 1; Split hand/foot malformation type 1 protein
Product Gene Name
SHFM1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P60896
Specificity
SHFM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SHFM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SHFM1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SHFM1 sirna
siRNA to inhibit SHFM1 expression using RNA interference
Applications Tested/Suitable for SHFM1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SHFM1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006295.1
[Other Products]
NCBI GenBank Nucleotide #
NM_006304.1
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UniProt Primary Accession #
P60896
[Other Products]
UniProt Secondary Accession #
Q13437; Q61067[Other Products]
UniProt Related Accession #
P60896[Other Products]
Molecular Weight
8,278 Da
NCBI Official Full Name
26S proteasome complex subunit DSS1
NCBI Official Synonym Full Names
split hand/foot malformation (ectrodactyly) type 1
NCBI Official Symbol
SHFM1??[Similar Products]
NCBI Official Synonym Symbols
ECD; DSS1; SEM1; SHFD1; SHSF1; Shfdg1
??[Similar Products]
NCBI Protein Information
26S proteasome complex subunit DSS1
UniProt Protein Name
26S proteasome complex subunit DSS1
UniProt Synonym Protein Names
Deleted in split hand/split foot protein 1; Split hand/foot deleted protein 1; Split hand/foot malformation type 1 protein
Protein Family
26S proteasome complex
UniProt Gene Name
SHFM1??[Similar Products]
UniProt Synonym Gene Names
DSS1; SHFDG1??[Similar Products]
UniProt Entry Name
DSS1_HUMAN
NCBI Summary for SHFM1
The product of this gene has been localized within the split hand/split foot malformation locus SHFM1 at chromosome 7. It has been proposed to be a candidate gene for the autosomal dominant form of the heterogeneous limb developmental disorder split hand/split foot malformation type 1. In addition, it has been shown to directly interact with BRCA2. It also may play a role in the completion of the cell cycle. [provided by RefSeq, Jul 2008]
UniProt Comments for SHFM1
SHFM1: Subunit of the 26S proteasome which plays a role in ubiquitin-dependent proteolysis. Belongs to the DSS1/SEM1 family.
Protein type: Proteasome complex; Protease; DNA repair, damage
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: proteasome complex; integrator complex
Molecular Function: protein binding
Biological Process: double-strand break repair via homologous recombination
Disease: Split-hand/foot Malformation 1
Research Articles on SHFM1
1. Breast cancers with high DSS1 expression have worse prognosis and shorter relapse-free survival times.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
