DCR1; MNG1; Dicer; HERNA; RMSE2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-DICER1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.

Polyclonal

Western Blot (WB), Immunoprecipitation (IP)

WB: 1:500 - 1:1000
IP: 1:20 - 1:50

Western blot analysis of extracts of various cell lines, using DICER1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody at dilution of 1:100 (40x lens).

1922

dicer 1, ribonuclease type III

endoribonuclease Dicer; helicase MOI; Dicer1, Dcr-1 homolog; helicase with RNAse motif; dicer 1, double-stranded RNA-specific endoribonuclease

Endoribonuclease Dicer

DICER; HERNA; KIAA0928; Helicase MOI??[Similar Products]

DICER_HUMAN

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.

Protein type: Helicase; Cell development/differentiation; Ribonuclease; RNA-binding; EC 3.1.26.3; RNA processing

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: growth cone; axon; cytoplasm; dendrite; ER-Golgi intermediate compartment; nucleus; cytosol

Molecular Function: endoribonuclease activity; protein binding; miRNA binding; ribonuclease III activity; metal ion binding; double-stranded RNA binding; deoxyribonuclease I activity; helicase activity; ATP binding

Biological Process: zygote asymmetric cell division; regulation of neuron differentiation; regulation of cell cycle; regulation of oligodendrocyte differentiation; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; olfactory bulb interneuron differentiation; post-embryonic development; cardiac muscle cell development; pre-microRNA processing; angiogenesis; DNA fragmentation during apoptosis; defense response to virus; regulation of viral genome replication; spleen development; positive regulation of myelination; positive regulation of Schwann cell differentiation; hair follicle morphogenesis; inner ear receptor cell development; mRNA stabilization; RNA interference, targeting of mRNA for destruction; multicellular organism growth; stem cell maintenance; RNA interference, conversion of ds siRNA to ss siRNA; spindle assembly; spinal cord motor neuron differentiation; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; branching morphogenesis of a tube; cartilage development; gene expression; nerve development; cerebral cortex development; RNA interference, production of siRNA; reproductive structure development; neurite morphogenesis; lung development; myoblast cell differentiation involved in skeletal muscle regeneration

Disease: Pleuropulmonary Blastoma; Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Rhabdomyosarcoma, Embryonal, 2

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.