Product Name
Amylin, ELISA Kit
Popular Item
Full Product Name
Rat Amylin ELISA Kit
Product Gene Name
AMYLIN elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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Chromosome Location
Chromosome: 12; NC_000012.11 (21525802..21532914). Location: 12p12.1
3D Structure
ModBase 3D Structure for P10997
Specificity
No significant cross-reactivity or interference between Rat AMYLIN and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Detection Range
0.25 ?mol/L - 8 ?mol/L.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AMYLIN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMYLIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AMYLIN and AMYLIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMYLIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of AMYLIN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AMYLIN purchase
MBS028823 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amylin, ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMYLIN. The ELISA analytical biochemical technique of the MBS028823 kit is based on AMYLIN antibody-AMYLIN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMYLIN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMYLIN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
AMYLIN elisa kit
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated AMYLIN concentrations in Rat serum, plasma and other body fluids. Using Purified Rat AMYLIN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AMYLIN and AMYLIN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AMYLIN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI/Uniprot data below describe general gene information for AMYLIN. It may not necessarily be applicable to this product.
NCBI Accession #
AAA35524.1
[Other Products]
UniProt Primary Accession #
P10997
[Other Products]
UniProt Secondary Accession #
Q0ZD87; Q14598[Other Products]
UniProt Related Accession #
P10997[Other Products]
Molecular Weight
9,806 Da
NCBI Official Full Name
amylin, partial
NCBI Official Synonym Full Names
islet amyloid polypeptide
NCBI Official Symbol
IAPP??[Similar Products]
NCBI Official Synonym Symbols
DAP; IAP
??[Similar Products]
NCBI Protein Information
islet amyloid polypeptide; amylin; insulinoma amyloid peptide; diabetes-associated peptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin)
UniProt Protein Name
Islet amyloid polypeptide
UniProt Synonym Protein Names
Amylin; Diabetes-associated peptide; DAP; Insulinoma amyloid peptide
UniProt Gene Name
IAPP??[Similar Products]
UniProt Synonym Gene Names
DAP??[Similar Products]
UniProt Entry Name
IAPP_HUMAN
NCBI Summary for AMYLIN
Islet, or insulinoma, amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Studies suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. [provided by RefSeq, Apr 2011]
UniProt Comments for AMYLIN
IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12p12.1
Cellular Component: extracellular space; cell soma; extracellular region
Molecular Function: identical protein binding; hormone activity; receptor binding
Biological Process: negative regulation of cell differentiation; cell-cell signaling; apoptosis; eating behavior; signal transduction; sensory perception of pain; negative regulation of bone resorption; endocrine pancreas development
Research Articles on AMYLIN
1. the presence of Ca(2+) ions inhibits membrane damage occurring immediately after the interaction of freshly dissolved hIAPP with the membrane.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
