Anti -Desmocollin-2,3

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 18; NC_000018.9 (28645942..28682388, complement). Location: 18q12.1

Monoclonal

IgG1

3G130

Mouse

Recognizes the ~120kD desmocollin-2 and ~105kD desmocollin-3 human protein, with particularly strong reaction to desmocollin-2. Crossreactivity with other desmocollin family members has not been observed.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-DSC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein1-3 and desmocollin1-3. Desmosomal cadherins showed differentiation- specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darier's disease. Desmosomal cadherin antibodies are also useful for targeting specific disease and in the study of interaction between cell adhesion proteins.

Antibodies; Abs to Proteins

Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence., Recommended Dilutions:, Immunoprecipitation (Native): 5-10ug, Western Blot (ECL): 1-3ug/ml, Immuno uorescence: 20-50ug/ml, Optimal dilutions to be determined by the researcher., Recommended Positive Control:, A0001-50: A431 Cells, Non-Stimulated

99,962 Da[Similar Products]

desmocollin 2

desmocollin-2; OTTHUMP00000162822; OTTHUMP00000162823; cadherin family member 2; desmosomal glycoprotein II; desmosomal glycoprotein III; desmosomal glycoprotein II/III

Desmocollin-2

CDHF2; DSC3??[Similar Products]

DSC2_HUMAN

The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11. Alternative splicing results in two transcript variants encoding distinct isoforms. [provided by RefSeq]

DSC2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Defects in DSC2 are the cause of familial arrhythmogenic right ventricular dysplasia type 11 (ARVD11); also known as arrhythmogenic right ventricular cardiomyopathy 11 (ARVC11). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; cell-cell adherens junction; plasma membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: protein binding; calcium ion binding

Biological Process: homophilic cell adhesion; cell adhesion; cellular response to starvation

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 11

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