Goat Anti-Desmoplakin (aa 1546-1558) Antibody; DSP; desmoplakin; DPI; 250kDa paraneoplastic pemphigus antigen; desmoplakin I; 250kDa paraneoplastic pemphigus antigen antibody; desmoplakin antibody; desmoplakin I antibody; DPI antibody; DSP antibody; Desmoplakin; Desmoplakin (aa 1672 -1684)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-DSP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6ug/ml.
Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml).

(3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

278,916 Da

desmoplakin

desmoplakin

Desmoplakin

DP??[Similar Products]

DESP_HUMAN

This gene encodes a protein that anchors intermediate filaments to desmosomal plaques and forms an obligate component of functional desmosomes. Mutations in this gene are the cause of several cardiomyopathies and keratodermas, including skin fragility-woolly hair syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Desmoplakin: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. Belongs to the plakin or cytolinker family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: cornified envelope; extracellular matrix; intermediate filament; nucleus; plasma membrane

Molecular Function: protein binding; protein binding, bridging; protein kinase C binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: epidermis development; intermediate filament organization; keratinocyte differentiation; peptide cross-linking

Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 8; Cardiomyopathy, Dilated, With Woolly Hair And Keratoderma; Cardiomyopathy, Dilated, With Woolly Hair, Keratoderma, And Tooth Agenesis; Epidermolysis Bullosa, Lethal Acantholytic; Keratosis Palmoplantaris Striata Ii; Skin Fragility-woolly Hair Syndrome

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