Anti -ROBO3 (Roundabout Homolog 3, Roundabout-like Protein 3)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 11; NC_000011.9 (124735305..124751370). Location: 11q24.2

Polyclonal

Goat

Recognizes human ROBO3. Species sequence homology: Bovine, canine, mouse, porcine and rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ROBO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Roundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development.

Antibodies; Abs to Neuroscience

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Western Blot: 0.5-1ug/ml, observed in human brain, cerebellum and hippocampus, lysates on ~150kD bands

148,209 Da[Similar Products]

roundabout, axon guidance receptor, homolog 3 (Drosophila)

roundabout homolog 3; roundabout-like protein 3; retinoblastoma inhibiting gene 1

Roundabout homolog 3

ROBO3_HUMAN

This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]

ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: axon; integral to membrane

Biological Process: axon guidance; neuron migration; axon midline choice point recognition

Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis

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