Mouse Gamma-aminobutyric acid receptor subunit alpha-1 (GABRA1) ELISA kit; ECA4; EJM; EJM5; ; gamma-aminobutyric acid (GABA) A receptor; alpha 1

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between Mouse GABRA1 and analogues was observed.

Store all reagents at 2-8 degree C

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Small volumes of GABRA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9339158 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the gamma-aminobutyric acid (GABA) A receptor, alpha 1 (GABRA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GABRA1. The ELISA analytical biochemical technique of the MBS9339158 kit is based on GABRA1 antibody-GABRA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GABRA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GABRA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GABRA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human GABRA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABRA1 and GABRA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

51,754 Da

gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1

gamma-aminobutyric acid receptor subunit alpha-1; GABAA alpha 1; GABAAR alpha1; GABA(A) receptor subunit alpha-1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1

Gamma-aminobutyric acid receptor subunit alpha-1

Gabra-1??[Similar Products]

GBRA1_MOUSE

GABRA1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4). A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into *****hood. Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5). A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

Protein type: Transporter; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Cellular Component: postsynaptic membrane; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction

Molecular Function: chloride channel activity; protein binding; GABA-A receptor activity; GABA receptor activity; ion channel activity; drug binding; extracellular ligand-gated ion channel activity

Biological Process: transport; synaptic transmission, GABAergic; ion transport; chloride transport; gamma-aminobutyric acid signaling pathway

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