Product Name
Desmoplakin 1 + 2 (DSP), Monoclonal Antibody
Popular Item
Full Product Name
Desmoplakin 1 + 2 antibody
Product Synonym Names
Monoclonal Desmoplakin 1 + 2; Anti-Desmoplakin 1 + 2; Desmoplakin 1/2
Product Gene Name
anti-DSP antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15924
Clone Number
DP 1 + 2-2.15
Species Reactivity
Bovine, Chicken, Human, Mouse, Rat
Specificity
Bovine desmoglein 1/2
Purity/Purification
Protein A affinity chromatography
Form/Format
Supplied as a lyophilized powder. Reconstitute in 1 ml dist. water (final solution contains 0.09 % NaN3, 0.5% BSA in PBS buffer, pH 7.4)
Biological Significance
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Desmoplakin 1/2 antibody was raised in mouse using bovine desmoplakin 1&2 as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-DSP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DSP antibody
Mouse monoclonal Desmoplakin 1 + 2 antibody
Product Categories/Family for anti-DSP antibody
Cell Biology
Applications Tested/Suitable for anti-DSP antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin, Western Blot (WB)
Application Notes for anti-DSP antibody
IHC-F: 1:10
NCBI/Uniprot data below describe general gene information for DSP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004406.2
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NCBI GenBank Nucleotide #
NM_004415.2
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UniProt Primary Accession #
P15924
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UniProt Secondary Accession #
O75993; Q14189; Q9UHN4; B2RTT2; D7RX09[Other Products]
UniProt Related Accession #
P15924[Other Products]
Molecular Weight
278,916 Da[Similar Products]
NCBI Official Full Name
desmoplakin isoform I
NCBI Official Synonym Full Names
desmoplakin
NCBI Official Symbol
DSP??[Similar Products]
NCBI Official Synonym Symbols
DP; DPI; DPII; DCWHKTA
??[Similar Products]
NCBI Protein Information
desmoplakin
UniProt Protein Name
Desmoplakin
UniProt Synonym Protein Names
250/210 kDa paraneoplastic pemphigus antigen
Protein Family
Desmoplakin
UniProt Gene Name
DSP??[Similar Products]
UniProt Synonym Gene Names
DP??[Similar Products]
UniProt Entry Name
DESP_HUMAN
NCBI Summary for DSP
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus. [provided by RefSeq, Jul 2008]
UniProt Comments for DSP
Desmoplakin: Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin- plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes. Homodimer. Interacts with COL17A1 (via cytoplasmic region). Associates (via C-terminal) with KRT5-KRT14 (via rod region), KRT8-KRT18 and VIM intermediate filaments. Interacts with DSC2. Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin. Belongs to the plakin or cytolinker family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p24
Cellular Component: desmosome; mitochondrion; basolateral plasma membrane; plasma membrane; intermediate filament; cornified envelope; fascia adherens; nucleus
Molecular Function: protein binding, bridging; protein binding; protein kinase C binding; structural constituent of cytoskeleton; cell adhesion molecule binding; structural molecule activity
Biological Process: keratinocyte differentiation; cell-cell adhesion; epidermis development; wound healing; apoptosis; intermediate filament organization; peptide cross-linking; cell structure disassembly during apoptosis
Disease: Cardiomyopathy, Dilated, With Woolly Hair And Keratoderma; Arrhythmogenic Right Ventricular Dysplasia, Familial, 8; Keratosis Palmoplantaris Striata Ii; Cardiomyopathy, Dilated, With Woolly Hair, Keratoderma, And Tooth Agenesis; Skin Fragility-woolly Hair Syndrome; Epidermolysis Bullosa, Lethal Acantholytic
Research Articles on DSP
1. Case Report: PKP2/DSP mutations in patient with Brugada syndrome and ventricular tachycardia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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