Product Name
58K Golgi protein (FTCD), Monoclonal Antibody
Full Product Name
Anti-58K Golgi protein Rabbit Monoclonal Antibody
Product Synonym Names
Formimidoyltransferase-cyclodeaminase; Formiminotransferase-cyclodeaminase; FTCD; LCHC1; Glutamate formimidoyltransferase; 2.1.2.5; Glutamate formiminotransferase; Glutamate formyltransferase; Formimidoyltetrahydrofolate cyclodeaminase; 4.3.1.4; Formiminotetrahydrofolate cyclodeaminase; FTCD
Product Gene Name
anti-FTCD antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O95954
Purity/Purification
Affinity-chromatography
Immunogen
A synthesized peptide derived from human 58K Golgi protein
Subcellular Localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus. More abundantly located around the mother centriole.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-FTCD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FTCD antibody
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Product Categories/Family for anti-FTCD antibody
Signal Transduction; Metabolism; Amino Acids; Tags & Cell Markers; Subcellular Markers; organelles; golgi; Metabolism; Pathways and Processes; Metabolic Signaling Pathways; Amino Acid Metabolism; Types of Disease; Cancer
Applications Tested/Suitable for anti-FTCD antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-FTCD antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
FC/FACS: 1:100
Western Blot (WB) of anti-FTCD antibody
Western blot analysis of 58K Golgi protein expression in human fetal liver lysate (MBS1750052).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTCD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FTCD
NCBI/Uniprot data below describe general gene information for FTCD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001307341.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001320412.1
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UniProt Primary Accession #
O95954
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UniProt Secondary Accession #
Q86V03; Q9HCT4; Q9HCT5; Q9HCT6; Q9UHJ2; B9EGD0[Other Products]
UniProt Related Accession #
O95954[Other Products]
Molecular Weight
58927 MW
NCBI Official Full Name
formimidoyltransferase-cyclodeaminase isoform C
NCBI Official Synonym Full Names
formimidoyltransferase cyclodeaminase
NCBI Official Symbol
FTCD??[Similar Products]
NCBI Official Synonym Symbols
LCHC1
??[Similar Products]
NCBI Protein Information
formimidoyltransferase-cyclodeaminase
UniProt Protein Name
Formimidoyltransferase-cyclodeaminase
UniProt Synonym Protein Names
Formiminotransferase-cyclodeaminase; FTCD; LCHC1Including the following 2 domains:Glutamate formimidoyltransferase (EC:2.1.2.5)Alternative name(s):Glutamate formiminotransferase; Glutamate formyltransferase
Protein Family
Formimidoyltransferase-cyclodeaminase
UniProt Gene Name
FTCD??[Similar Products]
UniProt Synonym Gene Names
FTCD??[Similar Products]
NCBI Summary for FTCD
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]
UniProt Comments for FTCD
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.Binds and promotes bundling of vimentin filaments originating from the Golgi.
Research Articles on FTCD
1. The formiminotransferase-cyclodeaminase allelic spectrum comprised of 12 distinct variants including 5 missense alterations, an in-frame deletion, two frameshift variants and four nonsense variants with the remaining alterations predicted to affect mRNA processing/stability.
Precautions
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Disclaimer
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