Product Name
Lactoferrin (LTF), ELISA Kit
Full Product Name
Canine Lactoferrin (LTF) ELISA Kit
Product Gene Name
LTF elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
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Species Reactivity
Canine
Specificity
No cross-reaction with other factors.
Samples
Canine serum, plasma or cell culture supernatant and organizations
Assay Type
Quantitative Sandwich
Detection Range
20 ng/ml-0.312 ng/ml
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of LTF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for LTF purchase
MBS2603271 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lactoferrin (LTF) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LTF. The ELISA analytical biochemical technique of the MBS2603271 kit is based on LTF antibody-LTF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LTF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LTF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
LTF elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine LTF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Typical Testing Data/Standard Curve (for reference only) of LTF elisa kit
NCBI/Uniprot data below describe general gene information for LTF. It may not necessarily be applicable to this product.
NCBI Accession #
AAB57795.1
[Other Products]
UniProt Secondary Accession #
O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; A8K9U8; B2MV13; B7Z4X2; E7EQH5[Other Products]
UniProt Related Accession #
P02788[Other Products]
Molecular Weight
73,161 Da
NCBI Official Full Name
lactoferrin, partial
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
LTF??[Similar Products]
NCBI Official Synonym Symbols
LF; HLF2; GIG12; HEL110
??[Similar Products]
NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
UniProt Synonym Protein Names
Growth-inhibiting protein 12; Talalactoferrin
Protein Family
Lactotransferrin
UniProt Gene Name
LTF??[Similar Products]
UniProt Synonym Gene Names
GIG12; LF; Lactoferrin; Lfcin-H??[Similar Products]
UniProt Entry Name
TRFL_HUMAN
NCBI Summary for LTF
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
UniProt Comments for LTF
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 3.4.21.-; Secreted; Secreted, signal peptide; Protease
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; nucleus; protein complex; secretory granule; specific granule
Molecular Function: DNA binding; heparin binding; iron ion binding; protein binding; protein serine/threonine kinase activator activity; serine-type endopeptidase activity
Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; cell redox homeostasis; cellular protein metabolic process; humoral immune response; innate immune response in mucosa; interaction with host; ion transport; iron assimilation by chelation and transport; negative regulation of apoptosis; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; proteolysis; regulation of cytokine production; regulation of tumor necrosis factor production; response to host immune response; retinal homeostasis; transcription, DNA-dependent
Research Articles on LTF
1. Both the hLF and hLZ were expressed in the mammary gland of bi-transgenic pigs, as detected by western blotting.Interestingly, pig milk containing hLF and hLZ had synergistic antimicrobial activity
Precautions
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Disclaimer
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