Product Name
TIRAP, siRNA
Full Product Name
TIRAP siRNA (Human)
Product Synonym Names
MAL; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2
Product Gene Name
TIRAP sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P58753
Specificity
TIRAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TIRAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TIRAP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TIRAP sirna
siRNA to inhibit TIRAP expression using RNA interference
Applications Tested/Suitable for TIRAP sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TIRAP. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001034750.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001039661.1
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UniProt Primary Accession #
P58753
[Other Products]
UniProt Secondary Accession #
Q56UH9; Q56UI0; Q8N5E5; B3KW65[Other Products]
UniProt Related Accession #
P58753[Other Products]
Molecular Weight
27,948 Da
NCBI Official Full Name
toll/interleukin-1 receptor domain-containing adapter protein isoform a
NCBI Official Synonym Full Names
toll-interleukin 1 receptor (TIR) domain containing adaptor protein
NCBI Official Symbol
TIRAP??[Similar Products]
NCBI Official Synonym Symbols
Mal; wyatt; BACTS1; MyD88-2
??[Similar Products]
NCBI Protein Information
toll/interleukin-1 receptor domain-containing adapter protein
UniProt Protein Name
Toll/interleukin-1 receptor domain-containing adapter protein
UniProt Synonym Protein Names
Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2
Protein Family
Toll/interleukin-1 receptor domain-containing adapter protein
UniProt Gene Name
TIRAP??[Similar Products]
UniProt Synonym Gene Names
MAL; TIR domain-containing adapter protein; MyD88-2??[Similar Products]
UniProt Entry Name
TIRAP_HUMAN
NCBI Summary for TIRAP
The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
UniProt Comments for TIRAP
TIRAP: an adapter protein involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, ERK1, ERK2 and JNK, resulting in cytokine secretion and the inflammatory response. Homodimerizes. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9. Genetic variations in TIRAP can influence susceptibility or resistance to infectious diseases, including invasive pneumococcal disease, malaria and tuberculosis. Three isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: nucleoplasm; intercellular bridge; endocytic vesicle; cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding, bridging; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein homodimerization activity; protein kinase C binding; protein heterodimerization activity
Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of JNK cascade; response to lipopolysaccharide; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; regulation of innate immune response; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-8 production; defense response to Gram-positive bacterium; cell surface receptor linked signal transduction; positive regulation of interleukin-15 production; toll-like receptor signaling pathway; positive regulation of B cell proliferation; innate immune response; myeloid cell differentiation; regulation of interferon-beta production; inflammatory response; toll-like receptor 4 signaling pathway
Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Bacteremia, Susceptibility To, 1; Mycobacterium Tuberculosis, Susceptibility To; Malaria, Susceptibility To
Research Articles on TIRAP
1. Evaluated the possible association between TIRAP rs1893352 and rs8177374 (S180L) gene polymorphisms and pulmonary tuberculosis in a sample of Iranian population.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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