Product Name
TRPM6, siRNA
Full Product Name
TRPM6 siRNA (Human)
Product Synonym Names
CHAK2; Transient receptor potential cation channel subfamily M member 6; Channel kinase 2; Melastatin-related TRP cation channel 6
Product Gene Name
TRPM6 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9BX84
Specificity
TRPM6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPM6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TRPM6 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TRPM6 sirna
siRNA to inhibit TRPM6 expression using RNA interference
Applications Tested/Suitable for TRPM6 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TRPM6. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001170781.1
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NCBI GenBank Nucleotide #
NM_001177310.1
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UniProt Primary Accession #
Q9BX84
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UniProt Secondary Accession #
Q6VPR8; Q6VPR9; Q6VPS0; Q6VPS1; Q6VPS2[Other Products]
UniProt Related Accession #
Q9BX84[Other Products]
Molecular Weight
65,083 Da
NCBI Official Full Name
transient receptor potential cation channel subfamily M member 6 isoform b
NCBI Official Synonym Full Names
transient receptor potential cation channel, subfamily M, member 6
NCBI Official Symbol
TRPM6??[Similar Products]
NCBI Official Synonym Symbols
HSH; HMGX; HOMG; CHAK2; HOMG1
??[Similar Products]
NCBI Protein Information
transient receptor potential cation channel subfamily M member 6
UniProt Protein Name
Transient receptor potential cation channel subfamily M member 6
UniProt Synonym Protein Names
Channel kinase 2; Melastatin-related TRP cation channel 6
Protein Family
Transient receptor potential cation channel subfamily
UniProt Gene Name
TRPM6??[Similar Products]
UniProt Synonym Gene Names
CHAK2??[Similar Products]
UniProt Entry Name
TRPM6_HUMAN
NCBI Summary for TRPM6
This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Apr 2010]
UniProt Comments for TRPM6
ChaK2: is an unusual bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. ChaK2 mutations cause hereditary hypomagnesemia and secondary hypocalcemia (HSH). The TRP voltage-independent calcium-permeable cation channels are divided into six subfa milies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Related to ChaK1, another Atypical alpha kinase fused to a TRP ion channel protein. Forms homodimers or heterodimers with ChaK1. Six alternatively spliced isoforms have been described, three of which lack the ion channel region.
Protein type: Kinase, protein; Protein kinase, atypical; Membrane protein, multi-pass; EC 2.7.11.1; Membrane protein, integral; Channel, cation; ATYPICAL group; Alpha family; ChaK subfamily
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: brush border membrane; apical plasma membrane; plasma membrane; integral to membrane
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; calcium channel activity; ATP binding
Biological Process: response to toxin; protein amino acid phosphorylation; transmembrane transport
Disease: Hypomagnesemia 1, Intestinal
Research Articles on TRPM6
1. N-Myc can promote neuroblastoma cell proliferation through up-regulation of the channel kinases TRPM6 and TRPM7
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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