Transient receptor potential cation channel subfamily M member 6; Channel kinase 2; Melastatin-related TRP cation channel 6; TRPM6; CHAK2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This TRPM6 (CHAK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-348 amino acids from the Central region of human TRPM6 (CHAK2).

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-TRPM6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CHAK2 is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. It is also upregulated in certain forms of cancer and could play a role in diabetes.

Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

CHAK2 Antibody (H332) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CHAK2 antibody detected the CHAK2 protein (arrow).

TRPM6(CHAK2) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRPM6 Antibody (CHAK2) (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

231708

transient receptor potential cation channel, subfamily M, member 6

transient receptor potential cation channel subfamily M member 6

Transient receptor potential cation channel subfamily M member 6

CHAK2??[Similar Products]

TRPM6_HUMAN

This gene is predominantly expressed in the kidney and colon, and encodes a protein containing an ion channel domain and a protein kinase domain. It is crucial for magnesium homeostasis, and plays an essential role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Mutations in this gene are associated with hypomagnesemia with secondary hypocalcemia. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Apr 2010]

ChaK2: is an unusual bifunctional protein containing a transient receptor potential (TRP) ion channel fused to a protein kinase of the Atypical alpha group. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. ChaK2 mutations cause hereditary hypomagnesemia and secondary hypocalcemia (HSH). The TRP voltage-independent calcium-permeable cation channels are divided into six subfa milies, two of which (TRPC and TRPM) have members that are widely expressed and activated by oxidative stress. Related to ChaK1, another Atypical alpha kinase fused to a TRP ion channel protein. Forms homodimers or heterodimers with ChaK1. Six alternatively spliced isoforms have been described, three of which lack the ion channel region.

Protein type: Kinase, protein; Membrane protein, multi-pass; Channel, cation; Protein kinase, atypical; EC 2.7.11.1; Membrane protein, integral; ATYPICAL group; Alpha family; ChaK subfamily

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: brush border membrane; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; calcium channel activity; ATP binding

Biological Process: response to toxin; transmembrane transport; protein amino acid phosphorylation

Disease: Hypomagnesemia 1, Intestinal

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