Product Name
CYP11B1, siRNA
Full Product Name
CYP11B1 siRNA (Human)
Product Synonym Names
S11BH; Cytochrome P450 11B1 mitochondrial; CYPXIB1; Cytochrome P-450c11; Cytochrome P450C11; Steroid 11-beta-hydroxylase
Product Gene Name
CYP11B1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15538
Specificity
CYP11B1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYP11B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYP11B1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYP11B1 sirna
siRNA to inhibit CYP11B1 expression using RNA interference
Applications Tested/Suitable for CYP11B1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYP11B1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000488.3
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NCBI GenBank Nucleotide #
NM_000497.3
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UniProt Primary Accession #
P15538
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UniProt Secondary Accession #
Q14095; Q4VAQ8; Q4VAQ9; Q9UML2[Other Products]
UniProt Related Accession #
P15538[Other Products]
Molecular Weight
49,750 Da
NCBI Official Full Name
cytochrome P450 11B1, mitochondrial isoform 1
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily B, polypeptide 1
NCBI Official Symbol
CYP11B1??[Similar Products]
NCBI Official Synonym Symbols
FHI; CPN1; CYP11B; P450C11
??[Similar Products]
NCBI Protein Information
cytochrome P450 11B1, mitochondrial
UniProt Protein Name
Cytochrome P450 11B1, mitochondrial
UniProt Synonym Protein Names
CYPXIB1; Cytochrome P-450c11; Cytochrome P450C11; Steroid 11-beta-hydroxylase (EC:1.14.15.4)
Protein Family
Cytochrome
UniProt Gene Name
CYP11B1??[Similar Products]
UniProt Synonym Gene Names
S11BH; Cytochrome P450C11??[Similar Products]
UniProt Entry Name
C11B1_HUMAN
NCBI Summary for CYP11B1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for CYP11B1
CYP11B1: Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB. Defects in CYP11B1 are the cause of adrenal hyperplasia type 4 (AH4). AH4 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short ***** stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). AH4 patients usually have hypertension. Defects in CYP11B1 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - C21-steroid hormone; Oxidoreductase; EC 1.14.15.4; Lipid Metabolism - androgen and estrogen; Mitochondrial
Chromosomal Location of Human Ortholog: 8q21
Cellular Component: mitochondrion; mitochondrial inner membrane
Molecular Function: steroid 11-beta-monooxygenase activity; iron ion binding; heme binding
Biological Process: steroid metabolic process; xenobiotic metabolic process; regulation of blood pressure; C21-steroid hormone biosynthetic process; immune response; glucocorticoid biosynthetic process; glucose homeostasis; aldosterone biosynthetic process; sterol metabolic process; cellular response to hormone stimulus
Disease: Glucocorticoid-remediable Aldosteronism; Adrenal Hyperplasia, Congenital, Due To Steroid 11-beta-hydroxylase Deficiency
Research Articles on CYP11B1
1. Mutations in the CYP11B1 gene are the cause of 11beta-hydroxylase deficiency.
Precautions
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