Product Name
CYP27A1, siRNA
Full Product Name
CYP27A1 siRNA (Rat)
Product Synonym Names
CYP27; Sterol 26-hydroxylase mitochondrial; 5-beta-cholestane-3-alpha.7-alpha.12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Product Gene Name
CYP27A1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P17178
Specificity
CYP27A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CYP27A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYP27A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYP27A1 sirna
siRNA to inhibit CYP27A1 expression using RNA interference
Applications Tested/Suitable for CYP27A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYP27A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_849178.2
[Other Products]
NCBI GenBank Nucleotide #
NM_178847.3
[Other Products]
UniProt Primary Accession #
P17178
[Other Products]
UniProt Secondary Accession #
Q64615; Q64639[Other Products]
UniProt Related Accession #
P17178[Other Products]
Molecular Weight
60,733 Da
NCBI Official Full Name
sterol 26-hydroxylase, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 27, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp27a1??[Similar Products]
NCBI Official Synonym Symbols
Cyp27; P450C27
??[Similar Products]
NCBI Protein Information
sterol 26-hydroxylase, mitochondrial
UniProt Protein Name
Sterol 26-hydroxylase, mitochondrial
UniProt Synonym Protein Names
5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Protein Family
Sterol 26-hydroxylase
UniProt Gene Name
Cyp27a1??[Similar Products]
UniProt Synonym Gene Names
Cyp27??[Similar Products]
UniProt Entry Name
CP27A_RAT
NCBI Summary for CYP27A1
mitochondrial enzyme with both vitamin D3 25-hydroxylase and cholesterol 26-hydroxylase activities [RGD, Feb 2006]
UniProt Comments for CYP27A1
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
Research Articles on CYP27A1
1. Data indicate that inhibition of CYP27A1 activity or knockdown and deletion of the Cyp27a1 gene induced adipocyte differentiation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
