EC 2.7.1.11; PFK; 6-phosphofructokinase; Phosphofructokinase I; Phosphohexokinase

For Research Use Only. Not for use in diagnostic procedures.

Shipped and store at 4 degree C for 6 months.

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Small volumes of Pfk assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Background: Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui?s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy. The Phosphofructokinase Microplate Assay Kit, PFK converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. NADH can be measured at a colorimetric readout at 340 nm.

Functional Assay

86,720 Da[Similar Products]

Phosphofructokinase

CG4001 gene product from transcript CG4001-RE

ATP-dependent 6-phosphofructokinase

ATP-PFK; Phosphofructokinase??[Similar Products]

PFKA_DROME

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

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