25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; CP27A; Cytochrome P-450C27/25; Cytochrome P450 27; mitochondrial; Sterol 26-hydroxylase; Sterol 27-hydroxylase; Vitamin D

For Research Use Only. Not for use in diagnostic procedures.

One 96-Well Plate

Store at 4 degree C.

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Small volumes of CYP27A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9500942 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 27A1 (CYP27A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP27A1. The ELISA analytical biochemical technique of the MBS9500942 kit is based on CYP27A1 antibody-CYP27A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP27A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP27A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Cell Based ELISA Kit

60 kDa

cytochrome P450 family 27 subfamily A member 1

sterol 26-hydroxylase, mitochondrial

Sterol 26-hydroxylase, mitochondrial

CYP27??[Similar Products]

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

CYP27A1: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity. Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX). CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.15; Lipid Metabolism - primary bile acid biosynthesis; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity

Biological Process: bile acid biosynthetic process; sterol metabolic process

Disease: Cerebrotendinous Xanthomatosis

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