12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase; 7-alpha; Cholestanetriol 26 monooxygenase; CP27; CP27A_HUMAN; CTX; CYP; CYP27; CYP27A1; Cytochrome P 450C27/25; Cytochrome P-450C27/25; Cytochrome P450 27; Cytochrome P450 27 mitochondrial; Cytochrome P450 family 27 subfamily A polypeptide 1; Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1; mitochondrial; Sterol 26 hydroxylase; Sterol 26 hydroxylase mitochondrial; Sterol 26-hydroxylase; Sterol 27 hydroxylase; Sterol 27-hydroxylase; Vitamin D(3) 25 hydroxylase; Vitamin D(3) 25-hydroxylase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Cytochrome P450 27A1 antibody detects endogenous levels of total Cytochrome P450 27A1

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

1mg/ml (lot specific)

Store at -20 degree C. Stable for 12 months from date of receipt.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.

Small volumes of anti-CYP27A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Function: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
Similarity: Belongs to the cytochrome P450 family.

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

WB: 1:500-1:1000
IF/ICC: 1:100-1:500

MBS9605027 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

Western blot analysis of extracts from HeLa cells, using Cytochrome P450 27A1 antibody.The lane on the left is treated with the antigen-specific peptide.

Observed: 60 kD
Predicted: 61 kDa

cytochrome P450 family 27 subfamily A member 1

sterol 26-hydroxylase, mitochondrial

Sterol 26-hydroxylase, mitochondrial

CYP27??[Similar Products]

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.

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