Product Name
BGrowth Hormone (bGH), Recombinant Protein
Popular Item
Full Product Name
Recombinant Bovine Growth Hormone
Product Synonym Names
GH Bovine; Growth Hormone Bovine Recombinant; BGH; BST; rBGH; rBST; Bovine Somatotropin; Bovine GH; Growth hormone; GH1
Product Gene Name
bGH recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01246
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The Bovine Growth Hormone protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH 8-9.
Sterile Filtered White lyophilized (freeze-dried) powder.
Concentration
1mg/ml (lot specific)
Solubility
It is recommended to reconstitute the lyophilized Bovine Growth Hormone in 0.4% NaHCO3 or sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Biological Activity
The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
Preparation and Storage
Lyophilized Bovine Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GH Bovine should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Other Notes
Small volumes of bGH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
bGH recombinant protein
Description: Growth Hormone Bovine Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.8 kDa. The GH Bovine Recombinant is purified by proprietary chromatographic techniques.
Introduction: GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
Product Categories/Family for bGH recombinant protein
CYTOKINES AND GROWTH FACTORS; Growth Factors; Growth Hormone
NCBI/Uniprot data below describe general gene information for bGH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_851339.1
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NCBI GenBank Nucleotide #
NM_180996.1
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UniProt Primary Accession #
P01246
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UniProt Secondary Accession #
Q28117; Q3LS73; A4GX96[Other Products]
UniProt Related Accession #
P01246[Other Products]
Molecular Weight
24,558 Da
NCBI Official Full Name
somatotropin
NCBI Official Symbol
GH1??[Similar Products]
NCBI Official Synonym Symbols
GH
??[Similar Products]
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
UniProt Synonym Protein Names
Growth hormone
UniProt Gene Name
GH1??[Similar Products]
UniProt Synonym Gene Names
GH??[Similar Products]
UniProt Entry Name
SOMA_BOVIN
UniProt Comments for bGH
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Research Articles on bGH
1. Whereas only moderate structural changes were observed with up-regulation of GH/IGF-I axis, disruption of the GH receptor had pronounced effects upon tendon ultra-structure.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
