Product Name
DDX4, Monoclonal Antibody
Popular Item
Full Product Name
Anti-DDX4 Mouse mAb
Product Synonym Names
VASA; MGC111074; DDX4
Product Gene Name
anti-DDX4 antibody
[Similar Products]
Product Synonym Gene Name
VASA; MGC111074; DDX4[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Immunogen
Purified recombinant fragment of human DDX4 expressed in E Coli.
Other Notes
Small volumes of anti-DDX4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DDX4 antibody
Entrez Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Applications Tested/Suitable for anti-DDX4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Western Blot (WB) of anti-DDX4 antibody
Western blot analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence (IF) of anti-DDX4 antibody
Immunofluorescence analysis of MSCs (left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red
Flow Cytometry (FC/FACS) of anti-DDX4 antibody
Flow cytometric analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).
Immunohistochemistry (IHC) of anti-DDX4 antibody
Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
NCBI/Uniprot data below describe general gene information for DDX4. It may not necessarily be applicable to this product.
NCBI Accession #
JAP00617.1
[Other Products]
UniProt Secondary Accession #
Q5M7Z3; Q86VX0; Q9NT92; Q9NYB1; A8K8Q2; B3KSF4; D6RDK4; E9PCD8[Other Products]
UniProt Related Accession #
Q9NQI0[Other Products]
NCBI Official Full Name
DDX4, partial
NCBI Official Synonym Full Names
DEAD-box helicase 4
NCBI Official Symbol
DDX4??[Similar Products]
NCBI Official Synonym Symbols
VASA
??[Similar Products]
NCBI Protein Information
probable ATP-dependent RNA helicase DDX4
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX4
UniProt Synonym Protein Names
DEAD box protein 4; Vasa homolog
Protein Family
Probable ATP-dependent RNA helicase
UniProt Gene Name
DDX4??[Similar Products]
UniProt Synonym Gene Names
VASA??[Similar Products]
NCBI Summary for DDX4
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Comments for DDX4
DDX4: May play a role in germ cell development. May play a role in sperm motility. Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL. Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes. Belongs to the DEAD box helicase family. DDX4/VASA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.4.13; Helicase
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: cytoplasm; perinuclear region of cytoplasm
Molecular Function: ATP-dependent RNA helicase activity
Biological Process: DNA methylation during gametogenesis; male meiosis; RNA secondary structure unwinding; sperm motility; spermatogenesis
Research Articles on DDX4
1. Data show that intrinsically disordered N terminus of DEAD-box RNA helicase Ddx4 forms organelles in HeLa cells and in vitro.
Precautions
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Disclaimer
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