Mouse Sterol 26-hydroxylase; mitochondrial (CYP27A1) ELISA kit; CP27; CTX; CYP27; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 26-hydroxylase; 5-beta-cholestane-3-alpha; 7-alpha; 12-alpha-triol 27-hydroxylase; cholestanetriol 26-monooxygenase; cytochrome P-45; cytochrome P450; family 27; subfamily A; polypeptide 1

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of CYP27A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9320147 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP27A1. The ELISA analytical biochemical technique of the MBS9320147 kit is based on CYP27A1 antibody-CYP27A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP27A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP27A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

60,720 Da

cytochrome P450, family 27, subfamily a, polypeptide 1

sterol 26-hydroxylase, mitochondrial; cytochrome P450 27; cytochrome P450, 27; cytochrome P450, 27a1; sterol 27-hydroxylase; cytochrome P-450C27/25; cholesterol 27 hydroxylase; vitamin D(3) 25-hydroxylase; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase

Sterol 26-hydroxylase, mitochondrial

CP27A_MOUSE

CYP27A1: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity. Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX). CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts. Belongs to the cytochrome P450 family.

Protein type: Mitochondrial; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; EC 1.14.13.15

Cellular Component: mitochondrial envelope; membrane; mitochondrion; mitochondrial inner membrane

Molecular Function: cholestanetriol 26-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; iron ion binding; metal ion binding; cholesterol 26-hydroxylase activity; heme binding; oxidoreductase activity; monooxygenase activity; vitamin D3 25-hydroxylase activity

Biological Process: cholesterol metabolic process

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